Hyper efficient separations device
The present technology relates to improved device and methods of use of insulator-based dielectrophoresis. This device provides a multi-length scale element that provides enhanced resolution and separation. The device provides improved particle streamlines, trapping efficiency, and induces laterally similar environments. Also provided are methods of using the device.
1 . A multi-length scale structure for use in an insulator-based dielectrophoresis device for separating at least one analyte from a fluid, the device including a fluid flow channel disposed on a substrate, the fluid flow channel being defined by a first wall, and a second wall spaced from the first wall, the multi-length scale structure comprising:
an insulating flow structure extending from the first wall toward the second wall thereby defining a constriction in the fluid flow channel between the first wall and the second wall, the insulating flow structure comprising a base section and a plurality of projections extending from the base section toward the second wall,
wherein the plurality of projections are elliptically shaped,
wherein a base height of the base section is greater than a projection height of each of the plurality of projections,
wherein the projection height is in a range of 50 nm to 50 μm, and
wherein the plurality of projections are structured to create uniform projection heights at a plateau of greatest constriction of the insulating flow structure, and
wherein the base section is elliptically shaped.
2 . The multi-length scale structure of claim 1 , wherein each projection has a first end at a first junction with the base section and a second end at a second junction with the base section, a length from the first end to the second end being in the range of 50 nm-100 μm.
3 . The multi-length scale structure of claim 1 , wherein the base section extends from an upstream junction with the first wall to a downstream junction with the first wall, and the plurality of projections only extend from the base section over a portion of a distance from the upstream junction to the downstream junction.
4 . The multi-length scale structure of claim 3 , wherein the plurality of projections only extend from the base section over one half or less of the distance from the upstream junction to the downstream junction.
5 . A multi-length scale structure for use in an insulator-based dielectrophoresis device for separating at least one analyte from a fluid, the device including a fluid flow channel disposed on a substrate, the fluid flow channel being defined by a first wall, and a second wall spaced from the first wall, the multi-length scale structure comprising:
an insulating flow structure extending from the first wall toward the second wall thereby defining a constriction in the fluid flow channel between the first wall and the second wall, the insulating flow structure comprising a base section and a plurality of projections extending from the base section toward the second wall,
wherein the plurality of projections are elliptically shaped,
wherein a base height of the base section is greater than a projection height of each of the plurality of projections,
wherein the projection height is in a range of 50 nm to 50 μm, and
wherein the plurality of projections are structured to create uniform projection heights at a plateau of greatest constriction of the insulating flow structure,
wherein the second wall includes a second insulating flow structure extending toward the first wall thereby further defining the constriction in the fluid flow channel between the first wall and the second wall, the second insulating flow structure comprising a second base section and a second plurality of projections extending from the second base section toward the first wall, and
wherein the second base section is elliptically shaped and the second plurality of projections are elliptically shaped.
6 . The multi-length scale structure of claim 5 , wherein each of the second plurality of projections has a first end at a first junction with the second base section and a second end at a second junction with the second base section, a length from the first end to the second end being in the range of 50 nm-100 μm.
7 . The multi-length scale structure of claim 5 , wherein the second base section extends from an upstream junction with the second wall to a downstream junction with the second wall, and the second plurality of projections only extend from the second base section over a portion of a distance from the upstream junction to the downstream junction.
8 . The multi-length scale structure of claim 7 , wherein the second plurality of projections only extend from the second base section over one half or less of the distance from the upstream junction to the downstream junction.
9 . A method of separating a first analyte from a second analyte, the method comprising:
(a) providing the multi-length scale structure of claim 1 ; and
(b) transporting a fluid including the first analyte and the second analyte through the constriction in the fluid flow channel.
10 . A method of separating a first analyte from a second analyte, the method comprising:
(a) providing the multi-length scale structure of claim 5 ; and
(b) transporting a fluid including the first analyte and the second analyte through the constriction in the fluid flow channel.