Cellular delivery methods
An isolated, non-naturally occurring cell-penetrating peptide (CPP) comprising the amino acid sequence: [SEQ ID NO: 1] RRSRTARAGRPGRNSSRPSAPR and sequences which have at least 60% similarity to SEQ ID NO: 1.
1 . A non-naturally occurring cell-penetrating peptide (CPP) comprising the amino acid sequence of:
(i) RRSRTARAGRPGRNSSRPSAPR (SEQ ID NO: 1); or
(ii) the amino acid sequence of SEQ ID NO: 1 with one conservative amino acid substitution.
2 . The non-naturally occurring CPP of claim 1 , further comprising one or more of the following modifications: a non-canonical amino acid, a fatty acid, a detectable label, a cholesterol group, or a reactive group.
3 . A cell comprising the non-naturally occurring CPP of claim 1 .
4 . A kit comprising (i) the non-naturally occurring CPP of claim 1 and (ii) instructions for use.
5 . A cell comprising the CPP of claim 2 .
6 . A kit comprising the CPP of claim 1 and (ii) instructions for use.
7 . A kit comprising the modified cell of claim 5 and (ii) instructions for use.
8 . The non-naturally occurring CPP of claim 1 , wherein the amino acid sequence of the CPP consists of SEQ ID NO: 1.
9 . The non-naturally occurring CPP of claim 1 , comprising the amino acid sequence of SEQ ID NO: 1.
10 . The non-naturally occurring cell-penetrating peptide (CPP) of claim 1 , wherein each of the amino acid residues of the amino acid sequence are D-amino acid residues.
11 . The non-naturally occurring cell-penetrating peptide (CPP) of claim 1 , wherein the amino acid sequence comprises the amino acid sequence of SEQ ID NO: 1 and each of the amino acid residues of SEQ ID NO: 1 are D-amino acid residues.
12 . The non-naturally occurring cell-penetrating peptide (CPP) of claim 8 , wherein each of the amino acid residues of the amino acid sequence are D-amino acid residues.
13 . The non-naturally occurring cell-penetrating peptide (CPP) of claim 2 , wherein each of the amino acid residues of the amino acid sequence are D-amino acid residues.