CAR-CD123 vector and uses thereof
The present invention relates to a chimeric antigen receptor (CAR) molecule containing, from the N-terminus to the C-terminus: a) an extracellular domain and transmembrane domain of human CD19, b) an antigen binding domain, c) a spacer domain, d) a transmembrane domain, and e) a cytoplasmatic domain, preferably wherein the CAR is a CD123 specific CAR (CD123 CAR) and the antigen binding domain includes a VL and/or VH from a monoclonal anti-CD123 antibody, more preferably the antigen binding domain includes a single chain variable fragment (scFv) that specifically binds to CD123.
1 . A chimeric antigen receptor (CAR) molecule comprising, the sequence of SEQ ID NO: 12.
2 . The CAR molecule according to claim 1 , that consists of the sequence of SEQ ID NO: 12.
3 . An isolated nucleic acid molecule encoding the chimeric antigen receptor molecule according to claim 1 , wherein said isolated nucleic acid molecule is operatively linked to expression control sequences.
4 . A vector comprising the isolated nucleic acid molecule according to claim 3 .
5 . An isolated engineered immune cell, comprising the vector according to claim 4 .
6 . A composition comprising the isolated nucleic acid molecule according to claim 3 , and a pharmaceutically acceptable excipient and/or adjuvant.
7 . A composition comprising a transduced cell comprising the CAR molecule of claim 1 .