IP Library › Granted Patent US 12698473
Granted Patent B2
US 12698473 · App. 17/935,846 · Granted Aug 4, 2026

Cell-containing structure

Inventors: Yuki Kaga (Yokohama, JP); Tomoaki Nakayama (Tokyo, JP); Tomoyuki Aratani (Yokohama, JP); Shinnosuke Koshizuka (Yamato, JP); Tatsuya Sameshima (Yokohama, JP); Momoko Shionoiri (Yokohama, JP); Tomofumi Kitazawa (Kawasaki, JP); Rie Ijichi (Tokyo, JP)
Assignee: Ricoh Company, Ltd.
C12N5/0619C12N2503/02C12N2506/45
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12698473
App. No.
17/935,846
Filed
Sep 27, 2022
Granted
Aug 4, 2026
Kind
B2
Art Unit
1634
USPC
435/368
Abstract

A cell-containing structure is provided that allows ready-to-use nerve drug response evaluation with high reproducibility to be easily performed. The cell-containing structure for evaluating an electrical property of neurons includes: (a) a culture surface to which the neurons are able to be adhered; (b) a cell mass that is adhered to the culture surface and contains at least one of the neurons; and (c) a plurality of electrodes for measuring the electrical property of the cell mass, wherein a spontaneous firing frequency of cells contained in the cell mass is 0.25 Hz or more per electrode.

Claims (34)

1 . A method for preparing a cell-containing structure for evaluating an electrical property of neurons, comprising:

1) providing a cell containing structures comprising

(a) a culture surface coated with a coating agent to which the neurons are able to be adhered;

(b) a cell mass that is adhered to the coated culture surface and contains the neurons; and

(c) a plurality of electrodes for measuring the electrical property of the cell mass,

2) determining a spontaneous firing frequency of cells contained in the cell mass and

3) retaining the cell-containing structures where the spontaneous firing frequency of cells contained in the cell mass is 0.25 Hz or more and less than 31.25 Hz, per electrode, thereby preparing a cell-containing structure for evaluating an electrical property of neurons.

2 . The method according to claim 1 ,

wherein the neurons are neurons obtained by in vitro differentiation.

3 . The method according to claim 1 ,

wherein the neurons are neurons derived from human artificial pluripotent stem cells (hiPSC).

4 . The method according to claim 3 ,

wherein the human artificial pluripotent stem cells are derived from healthy people.

5 . The method according to claim 3 ,

wherein the human artificial pluripotent stem cells are derived from a human having a disease.

6 . The method according to claim 5 ,

wherein the disease is a nervous disease.

7 . The method according to claim 5 ,

wherein the disease is autism, epilepsy, schizophrenia, ADHD, ALS or bipolar disorder.

8 . The method according to claim 1 ,

wherein the cell mass contains astrocytes.

9 . The method according to claim 1 ,

wherein the cell mass contains microglial cells or oligodendrocytes.

10 . The method according to claim 1 , further comprising a substrate which is an extracellular matrix.

11 . The method according to claim 10 ,

wherein the extracellular matrix contains laminin.

12 . The method according to claim 1 ,

wherein the electrical property is detected by a response to at least one stimulus treatment applied to the cell mass.

13 . The method according to claim 12 ,

wherein the stimulus treatment is exposure to at least one test compound.

14 . The method according to claim 13 ,

wherein the test compound is an agonist or antagonist of a neurotransmitter receptor.

15 . The method according to claim 13 ,

wherein the test compound is an ion channel blocker.