Formation of three-dimensional organ from pluripotent stem cells
View Patent ↗The present disclosure relates to an organ bud and a method of preparing an organ bud.
1 . An organ bud comprising iPSC-derived hepatocytes, vascular cells, and mesenchymal cells, wherein the hepatocytes are HNF4A positive.
2 . The organ bud of claim 1 , wherein the organ bud is vascularized.
3 . The organ bud of claim 1 , wherein the vascular cell includes a vascular endothelial cell.
4 . The organ bud of claim 1 , wherein the only cells cocultured to prepare the organ buds are the iPSC-derived hepatocytes, the vascular cells, and the mesenchymal cells.
5 . The organ bud of claim 1 , wherein the mesenchymal cell comprises a differentiated cell.
6 . The organ bud of claim 1 , wherein the mesenchymal cell comprises an undifferentiated cell.
7 . A method of preparing the organ bud of claim 1 , the method comprising culturing the iPSC-derived hepatocytes together with the vascular cells, and the mesenchymal cells.
8 . The method of claim 7 , wherein the iPSC-derived hepatocytes are cultured in a medium for culturing vascular cells, together with the mesenchymal cells.
9 . The method of claim 7 , wherein the iPSC-derived hepatocytes are plated on a gel and cultured together with the vascular cells, and the mesenchymal cells.
10 . A method of transplanting an organ bud, comprising transplanting the organ bud of claim 1 into a human or a non-human animal.
11 . The method of claim 10 , wherein the site of transplantation of the organ bud comprises at least one selected from the group consisting of intracranial space, mesenteric space, liver, spleen, kidney, kidney subcapsular space, and supraportal space.
12 . A method of regeneration or function recovery of a tissue or an organ, comprising transplanting the organ bud of claim 1 into a human or a non-human animal, wherein the organ bud is transplanted into at least one site selected from the group consisting of intracranial space, mesenteric space, kidney subcapsular site, and kidney.
13 . A method of preparing a non-human chimeric animal, comprising transplanting the organ bud of claim 1 into a non-human animal, wherein the organ bud is transplanted into at least one site selected from the group consisting of intracranial space, mesenteric space, kidney subcapsular site, and kidney.
14 . An organ bud comprising iPSC-derived transitional hepatic endoderm cells, vascular cells, and mesenchymal cells, wherein the iPSC-derived transitional hepatic endoderm cells are TBX3 positive and ADRA1B positive.
15 . A method of regeneration or function recovery of a tissue or an organ, comprising transplanting the organ bud of claim 1 into a human or a non-human animal, wherein the organ bud is transplanted into liver or spleen.
16 . A method of preparing a non-human chimeric animal, comprising transplanting the organ bud of claim 1 into a non-human animal, wherein the organ bud is transplanted into liver or spleen.