Electrochemical biosensor and the manufacturing method thereof
An electrochemical biosensor has an electrode which is sequentially decorated a molybdenum (VI) oxide (MoO 3 ) nanomaterial, a gold-composite nanomaterial, a carboxyl-sulfhydryl compound, and a carbodiimide/N-hydroxysuccinimide (EDC/NHS, EN) to modify the surface of the electrode. That the sulfhydryl group of the carboxyl-sulfhydryl compound forms a bond with the gold-composite nanomaterial, and the carbodiimide/N-hydroxybutanediimide forms a bond with a carboxyl group of the carboxyl-sulfhydryl compound.
1 . An electrochemical biosensor, consisting essentially of a working electrode with a surface provided with a molybdenum trioxide nanomaterial and a gold nanocomposite disposed over at least a portion of the molybdenum trioxide nanomaterial for modifying the working electrode, and a carboxyl-mercapto compound and a N-3-dimethylaminopropyl-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS, EN) for modification, wherein,
bonds form between a mercapto group of the carboxyl-mercapto compound and the gold nanocomposite, and the N-3-dimethylaminopropyl-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS, EN) bonds with a carboxyl group of the carboxyl-mercapto compound,
marker is attached to the N-3-dimethylaminopropyl-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS, EN), and
the gold nanocomposite is formed by gold nanoparticles coated on a surface of a metal oxide nanomaterial, wherein
the metal oxide nanomaterial comprises manganese dioxide (MnO2) or zinc oxide (ZnO).
2 . An electrochemical biosensor as claimed in claim 1 , wherein the shape of the metal oxide nanomaterials includes rods, flakes, and spheres or flowers.
3 . An electrochemical biosensor as claimed in claim 1 , wherein the carboxyl-mercapto compound is cysteine.
4 . An electrochemical biosensor as claimed in claim 2 , wherein the carboxyl-mercapto compound is cysteine.
5 . An electrochemical biosensor as claimed in claim 3 , wherein the marker is a primary antibody, and the primary antibody is a heat shock protein antibody, a calreticulin antibody, or a high mobility group box protein 1 antibody.
6 . An electrochemical biosensor as claimed in claim 4 , wherein the marker is a primary antibody, and the primary antibody is a heat shock protein antibody, a calreticulin antibody, or a high mobility group box protein 1 antibody.