IP Library › Granted Patent US 12699090
Granted Patent B2
US 12699090 · App. 17/602,628 · Granted Aug 4, 2026

Diagnostic reagents

Inventors: Vivek Kapur (State College, PA); Sreenidhi Srinivasan (State College, PA); Hans Vordermeier (Surrey, GB); Gareth Jones (Surrey, GB)
Assignees: The Penn State Research Foundation; The Secretary of State for Environment, Food and Rural Affairs acting through the Animal and Plant
G01N33/5695C07K14/35
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Quick Facts
Patent No.
US 12699090
App. No.
17/602,628
Granted
Aug 4, 2026
Kind
B2
Abstract

There is provided a skin test diagnostic reagent comprising: at least one 40mer polypeptide consisting of any one of SEQ ID NOs: 1, 3 or 4; at least one 40mer polypeptide consisting of any one of SEQ ID NOs: 7 or 8; and at least one 40mer polypeptide consisting of any one of SEQ ID NOs: 10, 11 or 12, characterised in that the reagent elicits a positive result when administered in a skin test to an animal infected with Mycobacterium bovis or Mycobacterium tuberculosis.

Claims (23)

1 . A skin test diagnostic reagent comprising:

a 40mer polypeptide consisting of SEQ ID NO: 1;

a 40mer polypeptide consisting of SEQ ID NO: 3;

a 40mer polypeptide consisting of SEQ ID NO: 4;

a 40mer polypeptide consisting of SEQ ID NO: 7;

a 40mer polypeptide consisting of SEQ ID NO: 8;

a 40mer polypeptide consisting of SEQ ID NO: 10;

a 40mer polypeptide consisting of SEQ ID NO: 11; and

a 40mer polypeptide consisting of SEQ ID NO: 12;

wherein the reagent elicits a positive result when administered in a skin test to an animal infected with Mycobacterium bovis or Mycobacterium tuberculosis.

2 . The skin test diagnostic reagent according to claim 1 , further comprising at least 1, 2, 3 or 4 further different polypeptides, each selected from:

a 40mer polypeptide consisting of SEQ ID NOs: 2, 5, 6 and 9; and

a 20mer polypeptide consisting of SEQ ID NO: 38.

3 . The skin test diagnostic reagent according to claim 1 , comprising each of the 40mer polypeptides consisting of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12 and the 20mer polypeptide consisting of SEQ ID NO: 38.

4 . The skin test diagnostic reagent according to claim 1 , comprising at least 1, 2 or 3 further different polypeptides, each selected from:

a 20mer polypeptide consisting of SEQ ID NOs: 17, 25, 36 and 39.

5 . The skin test diagnostic reagent according to claim 1 , comprising each of the 40mer polypeptides having SEQ ID NOs: 1, 3, 4, 7, 8, 10, 11 and 12, and each of the 20mer polypeptides having SEQ ID NOs: 17, 25, 36 and 39.

6 . The skin test diagnostic reagent according to claim 1 , for use in a method of detecting Mycobacterium bovis or Mycobacterium tuberculosis infection in an animal.

7 . A method of detecting Mycobacterium bovis or Mycobacterium tuberculosis infection in an animal comprising conducting a skin test on the animal using at least one skin test diagnostic reagent according to claim 1 .

8 . A diagnostic kit comprising the skin test diagnostic reagent according to claim 1 .

9 . The diagnostic kit according to claim 8 , wherein the diagnostic reagent is able to detect a Mycobacterium bovis or Mycobacterium tuberculosis infection in a mammal.

10 . The diagnostic kit according to claim 9 , wherein the diagnostic reagent is able to differentiate between a Mycobacterium bovis or Mycobacterium tuberculosis infected mammal and a mammal vaccinated against Mycobacterium bovis or Mycobacterium tuberculosis infection.

11 . The diagnostic kit according to claim 8 , wherein the diagnostic reagent is in liquid form and included in at least one aliquot of 0.05-0.15 ml containing 1-20 μg of each polypeptide contained in the skin test diagnostic reagent.