Biosensor and method for determination of viruses in coronavirus family
A method and a biosensor for determination of the presence of corona-virus are provided. The method includes qualitative or quantitative measurement of probable corona-virus presence by means of colorimetric and/or electrochemical transducer methods as a result of interaction of S-protein in patient samples by means of a biosensor including ACE2, DPP4 and CD147 bio-receptors as bio-receptor.
1 . A method for determining a presence of a corona-virus, comprising: qualitatively or quantitatively measuring the presence of the corona-virus in an analyte by an electrochemical transducer method by a biosensor including an ACE2 bio-receptor for a SARS virus and a SARS-2 virus, a DPP4 bio-receptor for a MERS virus, and a CD147 bio-receptor for the SARS-2 virus in a corona-virus family, wherein the method comprises the following process steps:
contacting the analyte, taken from a person in need, with the bio-receptors immobilized onto a working electrode,
conducting an interaction between the bio-receptors and S-proteins of the corona-virus existing in the analyte,
differentiating an impedance on a surface of the working electrode by binding of the S-proteins to the bio-receptors and determining whether the person in need is infected with a corona-virus by measuring a change.
2 . The method according to claim 1 , wherein gold and carbon based electrodes are used as the working electrode.
3 . The method according to claim 1 , wherein an immobilization process of the bio-receptors onto the surface of the working electrode takes place by an EDC/NHS cross-binder chemical compounds.
4 . A method for determining a presence of a corona-virus, comprising: quantitatively measuring the presence of the corona-virus in an analyte by a colorimetric transducer method by a biosensor including an ACE2 bio-receptor for a SARS virus and a SARS-2 virus, a DPP-4 bio-receptor for a MERS virus, and a CD147 bio-receptor for the SARS-2 virus in a corona-virus family, wherein the method comprises the following process steps: firstly adding a color forming and oxidizing reagent solution into an eppendorf tube to obtain a resulting tube, and afterwards adding a proper amount of the bio-receptors in the resulting tube to obtain a blue reacted bio-receptor solution,
adding the analyte obtained from a person in need into the blue reacted bio-receptor solution for mixing to lose a blue color as a result, wherein decolorization of the solution indicates the presence of corona-virus in the analyte; wherein γ-Fe 2 O 3 magnetic nanoparticles and a 3,3′,5,5′-tetramethylbenzidine (TMB) solution containing H 2 O 2 are used as the color forming and oxidizing reagent solution.
5 . The method according to claim 4 , wherein a binding region of a S-protein provided in a body of the corona-virus is bonded to the bio-receptors provided on a healthy cell surface, the blue reacted bio-receptor solution containing the H 2 O 2 oxidizes a thiol in a structure of a bio-receptor bound to the S-protein to a disulfide bond, and the blue reacted bio-receptor solution is reduced to lose the blue color.
6 . The method according to claim 1 , wherein the analyte is obtained by swabbing from a mouth and a nose of the person in need.
7 . The method according to claim 2 , wherein the analyte is obtained by swabbing from a mouth and a nose of the person in need.
8 . The method according to claim 3 , wherein the analyte is obtained by swabbing from a mouth and a nose of the person in need.
9 . The method according to claim 4 , wherein the analyte is obtained by swabbing from a mouth and a nose of the person in need.
10 . The method according to claim 5 , wherein the analyte is obtained by swabbing from a mouth and a nose of the person in need.