IP Library Granted Patent US 12699102
Granted Patent B2
US 12699102 · App. 18/015,210 · Granted Aug 4, 2026

Composition containing TARC, and method for improving storage stability of TARC

Inventors: Akiko Matsumoto (Tokyo, JP); Tomoyuki Usui (Tokyo, JP); Yuka Suzuki (Tokyo, JP); Kengo Fujimura (Tokyo, JP)
Assignee: SEKISUI MEDICAL CO., LTD.
G01N33/6863
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Quick Facts
Patent No.
US 12699102
App. No.
18/015,210
Granted
Aug 4, 2026
Kind
B2
Abstract

An object of the present invention is to provide a TARC-containing composition with high storage stability. Provided is a composition including TARC (Thymus and activation-regulated chemokine) and one or more selected from the group consisting of a sugar fatty acid ester-type nonionic surfactant, a polyoxyethylene-polyoxypropylene block copolymer-type nonionic surfactant, and a polyoxyethylene alkylamine-type nonionic surfactant and being in liquid form. The aforementioned object is achieved by the composition.

Claims (24)

1 . A composition comprising:

TARC (Thymus and activation-regulated chemokine); and

one or more selected from the group consisting of a sugar fatty acid ester-type nonionic surfactant, a polyoxyethylene-polyoxypropylene block copolymer-type nonionic surfactant, and a polyoxyethylene alkylamine-type nonionic surfactant, and

being in liquid form,

wherein the TARC concentration is 100 μg/mL to 50 ng/ml with respect to the composition, and

wherein the composition is filled in a storage container.

2 . The composition according to claim 1 , wherein the composition is a calibration sample solution for measuring TARC.

3 . The composition according to claim 1 , wherein the storage container is plastic.

4 . The composition according to claim 1 , wherein the concentration of the one or more selected from the group consisting of the sugar fatty acid ester-type nonionic surfactant, the polyoxyethylene-polyoxypropylene block copolymer-type nonionic surfactant, and the polyoxyethylene alkylamine-type nonionic surfactant is 0.00001 mass % to 1 mass % with respect to the composition.

5 . The composition according to claim 1 , wherein the sugar fatty acid ester-type nonionic surfactant is a polyoxyethylene sorbitan fatty acid ester-type nonionic surfactant.

6 . The composition according to claim 1 , wherein when the TARC concentration is 500 μg/mL with respect to the composition, the TARC residual rate is 80% or more after storage at 37° C. for 28 days in a plastic container.

7 . The composition according to claim 1 , wherein when the TARC concentration is 500 μg/mL with respect to the composition, the TARC residual rate is 90% or more after storage at 4° C. for 28 days in a plastic container.

8 . The composition according to claim 1 , wherein the sugar fatty acid ester-type nonionic surfactant is polyoxyethylene (20) sorbitan monolaurate, or polyoxyethylene (20) sorbitan monooleate; the polyoxyethylene-polyoxypropylene block copolymer-type nonionic surfactant is polyoxyethylene-polyoxypropylene condensate; and the polyoxyethylene alkylamine-type nonionic surfactant is polyoxyethylene (20) cured tallow amine.

9 . A kit for measuring TARC, wherein the kit comprises the composition according to claim 1 .

10 . A method for improving storage stability of TARC, comprising a step of contacting TARC with a solution containing one or more selected from the group consisting of a sugar fatty acid ester-type nonionic surfactant, a polyoxyethylene-polyoxypropylene block copolymer-type nonionic surfactant, and a polyoxyethylene alkylamine-type nonionic surfactant,

wherein the TARC concentration is adjusted so as to be 100 μg/mL to 50 ng/ml with respect to the solution.

11 . The method for improving the storage stability of TARC according to claim 10 , wherein a concentration of the one or more selected from the group consisting of a sugar fatty acid ester-type nonionic surfactant, a polyoxyethylene-polyoxypropylene block copolymer-type nonionic surfactant, and a polyoxyethylene alkylamine-type nonionic surfactant in the solution is 0.00001 mass % to 1 mass % with respect to the solution.

12 . The method for improving the storage stability of TARC according to claim 10 , wherein the sugar fatty acid ester-type nonionic surfactant is a polyoxyethylene sorbitan fatty acid ester-type nonionic surfactant.

13 . The method for improving the storage stability of TARC according to claim 10 , wherein the sugar fatty acid ester-type nonionic surfactant is polyoxyethylene (20) sorbitan monolaurate, or polyoxyethylene (20) sorbitan monooleate; the polyoxyethylene-polyoxypropylene block copolymer-type nonionic surfactant is polyoxyethylene-polyoxypropylene condensate; and the polyoxyethylene alkylamine-type nonionic surfactant is polyoxyethylene (20) cured tallow amine.

14 . A method for preventing adsorption of TARC in a solution containing TARC onto a container, comprising:

a step of adding a TARC adsorption inhibitor containing one or more selected from the group consisting of a sugar fatty acid ester-type nonionic surfactant, a polyoxyethylene-polyoxypropylene block copolymer-type nonionic surfactant, and a polyoxyethylene alkylamine-type nonionic surfactant, as active component, to the container, and

a step of adding TARC to the container.

15 . The method according to claim 14 , wherein the sugar fatty acid ester-type nonionic surfactant is a polyoxyethylene sorbitan fatty acid ester-type nonionic surfactant.

16 . The method according to claim 14 , wherein the sugar fatty acid ester-type nonionic surfactant is polyoxyethylene (20) sorbitan monolaurate, or polyoxyethylene (20) sorbitan monooleate; the polyoxyethylene-polyoxypropylene block copolymer-type nonionic surfactant is polyoxyethylene-polyoxypropylene condensate; and the polyoxyethylene alkylamine-type nonionic surfactant is polyoxyethylene (20) cured tallow amine.