Protein-enclosing polymeric micelle
The present invention provides a polymeric complex comprising a protein and a block copolymer represented by the following formula (1).
1 . A polymeric complex comprising a protein and a block copolymer represented by the following formula (1):
wherein R 1 represents an optionally substituted linear or branched alkyl group containing 1 to 12 carbon atoms and R 2 represents a hydrogen atom,
R 3 represents a compound represented by the following formula (Ia) or (Ib),
L 1 represents NH or a group represented by the following formula (11):
-(CH 2 ) p1 -NH- (11),
wherein p1 represents an integer of 1 to 6,
m1 and m2 each represent an integer of 0 to 500, provided that the sum of m1 and m2 represents an integer of 10 to 500, and with the proviso that m1 is not 0 and m2 is not 0,
m3, m4 and m5 each independently represent an integer of 1 to 5, and n represents an integer of 1 to 500, and
the symbol “/” means that (m1+m2) units of the respective monomer units shown on the left and right sides of this symbol may be in any sequence.
2 . The complex according to claim 1 , wherein the block copolymer represented by formula 1 is a block copolymer represented by the following formula (2),
3 . The complex according to claim 1 , wherein the protein is covalently bonded to the block copolymer represented by formula 1.
4 . The complex according to claim 3 , wherein the covalent bond is cleaved in a pH-dependent manner.
5 . A protein delivery device comprising the polymeric complex according to claim 1 for use in protein delivery to any site selected from a cell surface site, an intracellular site and an extracellular site.
6 . A protein delivery kit comprising a block copolymer represented by the following formula (1) for use in protein delivery to any site selected from a cell surface site, an intracellular site and an extracellular site:
wherein R 1 represents an optionally substituted linear or branched alkyl group containing 1 to 12 carbon atoms and R 2 represents a hydrogen atom,
R 3 represents a compound represented by the following formula (Ia) or (Ib),
L 1 represents NH or a group represented by the following formula (11):
—(CH 2 ) p1 -NH- (11)
wherein p1 represents an integer of 1 to 6,
m1 and m2 each independently represent an integer of 0 to 500 provided that the sum of m1 and m2 represents an integer of 1 to 500 and with the proviso that m1 is not 0 and m2 is not 0,
m3, m4 and m5 each independently represent an integer of 1 to 5, and n represents an integer of 1 to 500, and
the symbol “/” means that (m1+m2) units of the respective monomer units shown on the left and right sides of this symbol may be in any sequence.
7 . The kit according to claim 6 , wherein the block copolymer represented by formula 1 is a block copolymer represented by the following formula (2),