Antioxidant culture method and antioxidant auxiliary equipment
The present invention relates to an antioxidant culture method and antioxidant auxiliary equipment. The antioxidant cultivation method comprises: providing an electrolytic hydrogen generator comprising an anode, a cathode and a membrane between the cathode and the anode, the electrolytic hydrogen generator being connected with a bioreactor; inputting the culture medium in the bioreactor to the cathode and inputting a pure water to the anode; and providing an electric current to the electrolytic hydrogen generator to output a hydrogen from the cathode so as to dissolve the hydrogen in the culture medium of the bioreactor.
1 . An antioxidant culture method comprising:
providing a bioreactor containing cultured cells;
providing an electrolysis-based hydrogen generator comprising an anode, a cathode, and a septum between the anode and the cathode;
connecting the electrolysis-based hydrogen generator and the bioreactor;
inputting a culture medium of the bioreactor to the cathode and inputting a pure water to the anode; and
providing a current to the electrolysis-based hydrogen generator to output a hydrogen from the cathode and dissolve the hydrogen in the culture medium of the bioreactor;
wherein the method is applied in a virus antigen manufacturing procedure.
2 . The antioxidant culture method of claim 1 , further comprising: measuring a hydrogen concentration of the culture medium, and raising an activation frequency of the current when the hydrogen concentration is lower than a default value.
3 . The antioxidant culture method of claim 1 , wherein an activation frequency of the current is 1 to 3 times per hour for more than 10 minutes each time.
4 . The antioxidant culture method of claim 1 , wherein a hydrogen concentration of the culture medium ranges from 0.1 to 1.6 ppm.
5 . The antioxidant culture method of claim 1 , wherein providing the current to the electrolysis-based hydrogen generator comprises two stages: a first stage before infection of virus and a second stage after infection of virus, wherein the first stage and the second stage have different activation frequency of the current.
6 . The antioxidant culture method of claim 5 , wherein the activation frequency of the current at the second stage is higher than that at the first stage.
7 . The antioxidant culture method of claim 6 , wherein the activation frequency of the current at the second stage is twice the activation frequency of the current at the first stage.
8 . The antioxidant culture method of claim 7 , wherein the activation frequency of the current at the first stage is once an hour for 15 minutes each time, and the activation frequency of the current at the second stage is twice an hour for 15 minutes each time.
9 . The antioxidant culture method of claim 5 , wherein a duration of the first stage is proportional to a duration of the second stage.
10 . The antioxidant culture method of claim 9 , wherein the duration of the first stage and the duration of the second stage are 1 day.
11 . The antioxidant culture method of claim 1 , wherein the bioreactor is a tidal bioreactor and a default value of hydrogen concentration is 0.7 ppm.
12 . The antioxidant culture method of claim 1 , wherein the current is a constant current and does not exceed 0.5 ampere.
13 . The antioxidant culture method of claim 1 , wherein providing an electrolysis-based hydrogen generator is implemented by connecting an auxiliary equipment with the bioreactor, wherein the auxiliary equipment comprises:
the electrolysis-based hydrogen generator, the cathode being connected with the bioreactor, and the culture medium of the bioreactor being inputted into the electrolysis-based hydrogen generator via a first pump and hydrogen gas being outputted from the cathode to the bioreactor;
a pure water supply end connected with the anode, pure water in the pure water supply end being inputted into the electrolysis-based hydrogen generator via a second pump; and
a power control unit electrically connected with the electrolysis-based hydrogen generator for providing direct current to it and controlling the operation of the first pump and the second pump.
14 . The antioxidant culture method of claim 13 , wherein the cathode comprises a first pipeline and a second pipeline, the first pipeline and the second pipeline are connected with the bioreactor and the first pipeline is provided with the first pump, the second pipeline is used for outputting hydrogen gas, the anode comprises a third pipeline and a fourth pipeline, the third pipeline and the fourth pipeline are connected with the pure water supply end, the third pipeline is provided with a second pump and the fourth pipeline is used for outputting oxygen gas.
15 . The antioxidant culture method of claim 13 , wherein the cathode further comprises a cathode contact and the anode further comprises an anode contact, the anode contact and the cathode contact are electrically connected to the power control unit.
16 . The antioxidant culture method of claim 15 , wherein the power control unit comprises a power supply unit and a controller, a positive electrode of the power supply unit is electrically connected to the anode contact via the controller and the cathode contact is electrically connected to a negative electrode of the power supply unit, the controller is electrical connected with the first pump and the second pump.
17 . The antioxidant culture method of claim 13 , wherein the auxiliary equipment further comprises a hydrogen concentration detector which is arranged in the culture medium in the bioreactor and is connected to the power control unit for measuring the hydrogen concentration of the culture medium and adjusting the power control unit according to a comparison result of the hydrogen concentration of the culture medium and a default value.
18 . The antioxidant culture method of claim 17 , wherein the hydrogen concentration detector is a dissolved hydrogen meter.
19 . The antioxidant culture method of claim 13 , wherein the electrolysis-based hydrogen generator is a proton exchange membrane fuel cell, and the septum is a polymer membrane.