Microscope objective and microscope observation system

1 . A microscope objective, comprising:

a first movable lens group that is movable along an optical axis;

a first correction means configured to move the first movable lens group along the optical axis to correct axial chromatic aberration;

a second movable lens group that is movable along the optical axis; and

a second correction means configured to move the second movable lens group along the optical axis to correct spherical aberration,

wherein the first movable lens group is composed of a cemented doublet with a plano-convex lens and a plano-concave lens, and

the plano-convex lens and the plano-concave lens satisfy following conditional expressions (1) and (2):

❘

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n

1

-

n

2

❘

"\[RightBracketingBar]"

<

0.05

;

(

1

)

❘

"\[LeftBracketingBar]"

v

1

-

v

2

❘

"\[RightBracketingBar]"

>

10

;

(

2

)

n 1 : refractive index of the plano-convex lens on a reference wavelength;

n 2 : refractive index of the plano-concave lens on the reference wavelength;

v 1 : Abbe number of the plano-convex lens;

v 2 : Abbe number of the plano-concave lens.

2 . A microscope observation system, comprising:

the microscope objective according to claim 1 ;

an integrated workstation configured to control the first correction means and the second correction means of the microscope objective;

a tube lens unit configured to form an image in combination with the microscope objective; and

an image acquisition device configured to acquire the image formed by the tube lens unit,

wherein the integrated workstation is configured to

calculate a correction amount using image data acquired by the image acquisition device, and

control the first correction means and the second correction means based on the calculated correction amount.

3 . The microscope objective according to claim 1 , wherein, at the reference wavelength, the first movable lens group behaves substantially as a parallel plane plate, such that movement of the first movable lens group changes the axial chromatic aberration without substantially changing the spherical aberration, and movement of the second movable lens group corrects the spherical aberration without substantially changing the axial chromatic aberration.