Colony counting device, and control method
Counting accuracy of colonies is improved. A colony counting device captures a test individual to generate an image of the test individual, registers the image of the test individual in an initial state as a reference image, identifies a residue included in the reference image and identifies a position of the residue in a test image corresponding to the residue included in the reference image based on a displacement of each of two or more common characteristic portions between the reference image and the test image that is an image of the test individual after culturing, and counts the number of colonies while excluding the residue from a count as a colony based on the position of the residue.
1 . A colony counting device comprising:
an imaging section that captures a test individual and generates an image of the test individual;
an illumination section that illuminates the test individual;
a setting section that sets a test condition in at least one of the imaging section and the illumination section, the test condition including at least one of an imaging condition of the imaging section and an illumination condition of the illumination section;
a registration section that registers the image of the test individual in an initial state generated by the imaging section as a reference image;
a storage section that stores, in association with identification information of the test individual, (i) the reference image and (ii) the test condition applied to the at least one of the imaging section and the illumination section when the test individual in the initial state was captured when the reference image is registered by the registration section;
a receiving section that receives an input of the identification information of the test individual for reading the reference image registered by the registration section and for reading the test condition associated with the reference image,
wherein the setting section is configured to read, from the storage section, the test condition associated with the identification information of the test individual received by the receiving section after culturing of the test individual, and set the read test condition in the at least one of the imaging section and the illumination section;
a residue identifying section that identifies a residue included in the reference image registered by the registration section and associated with the identification information of the test individual received by the receiving section, and identifies a position of the residue in a test image being captured by the imaging section according to the test condition set by the setting section, corresponding to the residue included in the reference image based on a displacement of each of two or more common characteristic portions between the reference image and the test image that is generated by the imaging section and is an image of the test individual after culturing; and
a counting section that counts a number of colonies included in the test image while excluding image data at the identified position of the residue from a count as a colony based on the position of the residue identified by the residue identifying section.
2 . The colony counting device according to claim 1 ,
wherein the setting section is configured to set the illumination condition of the illumination section,
the registration section is configured to cause the storage section to store the illumination condition set in the illumination section in association with identification information of the test individual when the test individual before culturing is captured,
the setting section is configured to read, from the storage section, the illumination condition associated with the identification information of the test individual received again by the receiving section after culturing the test individual, and set the illumination condition of the illumination section, and
the imaging section is configured to capture the test individual in a state in which the test individual after culturing is illuminated according to the illumination condition set by the setting section and acquire the test image for counting the colonies.
3 . The colony counting device according to claim 1 , wherein
the residue identifying section recognizes a marker given on a peripheral edge portion of an upper surface or a lower surface of a cylindrical transparent container accommodating the test individual as at least one characteristic portion of the two or more common characteristic portions.
4 . The colony counting device according to claim 3 , wherein
the residue identifying section recognizes the marker having a linear shape as the at least one characteristic portion.
5 . The colony counting device according to claim 3 , wherein
the cylindrical transparent container includes an inner transparent container which has a bottomed cylindrical shape and in which a culture medium is formed, and a cylindrical outer transparent container serving as a lid of the inner transparent container, and
the residue identifying section recognizes a marker given on a peripheral edge portion of a bottom surface of the inner transparent container as the at least one characteristic portion of the two or more common characteristic portions.
6 . The colony counting device according to claim 1 , wherein
the residue identifying section recognizes a container accommodating the test individual, a residue adhering to the test individual, or a part of the test individual as at least one characteristic portion of the two or more common characteristic portions.
7 . The colony counting device according to claim 1 , further comprising a display section that displays the reference image acquired from the test individual before culturing and a moving image of the test individual after culturing acquired by the imaging section in a superimposed manner.
8 . The colony counting device according to claim 7 , further comprising:
a stage on which the test individual is placed; and
an alignment member that is provided on the stage and assists manual alignment by a user with respect to the test individual placed on the stage,
wherein the alignment member has a shape formed to come into contact with a container accommodating the test individual at at least two points to support the container.
9 . The colony counting device according to claim 1 , further comprising:
a table management section that creates a count table including a cell to which a count result of each of a plurality of the test individuals is input and stores the count table in a storage section;
a display section that displays the count table created by the table management section; and
a cell identifying section that identifies a target cell from among a plurality of the cells included in the count table displayed on the display section,
wherein the registration section that registers identification information of the test individual and the reference image in association with the target cell, and
the counting section is configured to read the reference image associated with the target cell from the storage section when the target cell is identified, count the number of colonies included in the test image while reducing the residue included in the reference image, and input or associate the counted number of colonies to or with the target cell.
10 . The colony counting device according to claim 9 , wherein
the count table includes a first cell and a second cell for each of the test individuals,
the first cell indicates whether or not the reference image has been acquired,
the second cell is associated with the first cell and indicates the number of colonies, and
the target cell is the second cell.
11 . The colony counting device according to claim 10 , wherein
the imaging section
captures an image of the test individual before culturing to acquire the reference image when the first cell is clicked, and
captures an image of the test individual after culturing using an imaging condition associated with the first cell to acquire the test image when the second cell is clicked.
12 . The colony counting device according to claim 1 , wherein
the counting section has a first mode in which an influence of the residue is mitigated and a second mode in which the influence of the residue is not mitigated,
the colony counting device further comprising a display section that displays an icon for selecting the first mode or the second mode.
13 . The colony counting device according to claim 1 , further comprising:
a designation section that designates the reference image from among a plurality of candidate images stored in the storage section; and
a display control section that reads at least one piece of information of a type of a culture medium, a dilution factor, a name of the test individual, and the identification information of the test individual associated with the reference image designated by the designation section from the storage section, and causes a display section to display the at least one piece of information.
14 . The colony counting device according to claim 13 , wherein
the identification information of the test individual is identification information of a container accommodating the test individual.
15 . The colony counting device according to claim 1 , wherein
the residue identifying section
identifies a position of the residue in the reference image, and
obtains relative displacements between a position and a rotation angle of the test image and a position and a rotation angle of the reference image based on the two or more common characteristic portions, and
the counting section is configured to
set a mask area in the test image based on the position of the residue and the relative displacements, and
count the number of colonies from the test image in which the mask area is set.
16 . A colony counting device comprising:
an illumination section that illuminates a test individual;
an imaging section that captures an image of the test individual to acquire a reference image for reducing an influence of a residue of the test individual, and captures an image of the test individual after culturing by the imaging section according to a test condition set by a setting section, which is applied to at least one of the imaging section and the illumination section, to acquire a test image for counting colonies;
the setting section that sets the test condition in the at least one of the imaging section and the illumination section, the test condition including at least one of an imaging condition of the imaging section and an illumination condition of the illumination section;
a storage section that stores, in association with identification information of the test individual, (i) the reference image and (ii) the test condition applied to the at least one of the imaging section and the illumination section when the test individual was captured;
a receiving section that receives an input of the identification information of the test individual for reading the reference image and for reading the test condition associated with the reference image,
wherein the setting section is configured to read, from the storage section, the test condition associated with the identification information of the test individual received by the receiving section after culturing of the test individual, and set the read test condition in the at least one of the imaging section and the illumination section; and
a counting section that counts a number of colonies in the reference image and a number of colonies in the test image being captured according to the test condition associated with the reference image, and subtracts the number of colonies in the reference image from the number of colonies in the test image to obtain a number of colonies in the test individual after culturing.
17 . A control method of a colony counting device, comprising:
capturing an image of a test individual to acquire a reference image for reducing an influence of a residue of the test individual;
storing, in association with identification information of the test individual, the reference image and a test condition applied to at least one of an imaging section and an illumination section when the test individual was captured, the test condition including at least one of an imaging condition of the imaging section and an illumination condition of the illumination section;
receiving the identification information of the test individual;
reading, based on the received identification information, the test condition associated with the reference image from a storage section and setting the read test condition in the at least one of the imaging section and the illumination section;
capturing an image of the test individual after culturing by the imaging section according to the set test condition to acquire a test image for counting colonies;
identifying the residue included in the reference image which is associated with the received identification information of the test individual;
identifying a position of the residue in the test image corresponding to the residue included in the reference image based on a displacement of each of two or more common characteristic portions between the reference image and the test image; and
counting a number of colonies included in the test image while excluding the residue from a count as a colony based on the identified position of the residue.