Soft tissue adhesive composition of alpha-TCP and phosphorylated amino acid

1 . A method of adhering a first soft tissue to a second soft tissue using a tissue adhesive comprising an aqueous solution, α-TCP, and a phosphorylated amino acid, wherein the tissue adhesive does not comprise a silicate compound, wherein the method comprises:

a. applying the tissue adhesive to the first soft tissue or to the second soft tissue and optionally leave it for a suitable period of time;

b. bringing the first tissue and the second tissue into contact with each other;

c. optionally applying a pressure on the first and second tissue for a suitable period of time; and

d. letting the tissue adhesive cure;

wherein the amount of phosphorylated amino acid is 40-90 wt % of the solid content.

2 . The method according to claim 1 wherein the steps are performed in vitro.

3 . The method according to claim 1 wherein the amount of the phosphorylated amino acid is 60-80 wt %.

4 . The method according to claim 1 wherein at least 95 wt % of the tissue adhesive comprises the aqueous solution, α-TCP and the phosphorylated amino acid.

5 . The method according to claim 1 wherein at least 98 wt % of the tissue adhesive comprises the aqueous solution, α-TCP and the phosphorylated amino acid.

6 . The method according to claim 1 wherein at least 98 wt % of the tissue adhesive comprises the aqueous solution, α-TCP and wherein the amount of phosphorylated amino acid is 60-80 wt %.

7 . The method according to claim 1 wherein the phosphorylated amino acid is phosphorylated serine.

8 . The method according to claim 1 wherein the aqueous solution is water.

9 . The method according to claim 1 wherein the tissue adhesive further comprises a hydrogel.

10 . The method according to claim 1 wherein the tissue adhesive further comprises collagen.

11 . The method according to claim 1 wherein at least one of the first and the second soft tissue is selected from tendon, ligament, fascia, skin, fibrous tissue, muscle, fat, nerve or blood vessel.