Formulation comprising a phosphocalcic cement and a physical and/or covalent hydrogel of polysaccharides, printable and having ductile mechanical properties for bone regeneration/bone repair
The present invention relates to the use of a formulation comprising a phosphocalcic cement and a physical and/or covalent hydrogel of polysaccharides for 3D printing, more particularly for bone regeneration and/or bone repair. The present invention also relates to a kit for 3D printing of bone implants comprising a phosphocalcic cement and a physical and/or covalent hydrogel of polysaccharides as well as to a method to prepare a formulation for 3D printing comprising a step of mixing a phosphocalcic cement and a physical and/or covalent liquid hydrogel precursor of polysaccharides.
1 . A method for 3D printing comprising the step of printing a formulation comprising a phosphocalcic cement and a physical and/or covalent hydrogel of polysaccharides.
2 . The method according to claim 1 , wherein said polysaccharides are hyaluronic acid and/or chitosan.
3 . The method according to claim 1 , wherein said hydrogel is a physical hydrogel.
4 . The method according to claim 1 , wherein said cement is a tricalcium phosphate cement.
5 . The method according to claim 1 , wherein the ratio hydrogel/cement is of 2:3 w/w.
6 . The method according to claim 1 , wherein said hydrogel is a physical hydrogel comprising from 2 to 4% w/v of hyaluronic acid and/or chitosan.
7 . The method according to claim 1 , wherein said hydrogel is a covalent hydrogel of silated hyaluronic acid and/or silated chitosan.
8 . The method according to claim 1 , for bone regeneration and/or bone repair.
9 . The method according to claim 1 in the context of vertical bone augmentation, complex geometries, large critical size bone defects, or following congenital diseases or to fill bone defects following tumor resections.
10 . The method according to claim 1 for 3D printing of bone implants.
11 . The method according to claim 1 , wherein the phosphocalcic cement is prepared with α-TCP.
12 . A kit for 3D printing of bone implants comprising:
(i) a phosphocalcic cement; and
(ii) a physical and/or covalent hydrogel of polysaccharides.
13 . A method to prepare a formulation for 3D printing comprising a step of mixing a phosphocalcic cement and a physical and/or covalent liquid hydrogel precursor of polysaccharides.
14 . An implant comprising a formulation as defined in claim 1 .
15 . Implant according to claim 14 , further comprising active molecules.
16 . The implant according to claim 14 , further comprising growth factors and/or cells.
17 . A method for bone repair and/or bone regeneration comprising:
(i) preparing a formulation comprising a phosphocalcic cement and a physical and/or covalent hydrogel of polysaccharides/or chitosan;
(ii) 3D printing of said formulation to form an implant; and optionally
(iii) inserting the implant obtained further to step (ii) in a bone cavity.
18 . A method according to claim 17 , wherein the cement and the hydrogel are sterilized in a sterile environment or wherein the implant is sterilized before insertion.
19 . The method for bone repair and/or bone regeneration according to claim 17 , comprising preparing a formulation comprising α-TCP powder, and a physical and/or covalent hydrogel of polysaccharides as in step (i).
20 . The method according to claim 17 , wherein the hydrogel comprises hyaluronic acid and/or chitosan.