IP Library Granted Patent US 12709733
Granted Patent B2
US 12709733 · App. 17/776,336 · Granted Aug 18, 2026

Cell culture media

Inventors: Aline Zimmer (Darmstadt, DE); Ronja Seibel (Darmstadt, DE); Corinna Schmidt (Darmstadt, DE); Gregor Franz Werner Wille (Buchs, CH); Markus Klaus Robert Fischer (Buchs, CH); Yannick Pierre Rey (Buchs, CH)
Assignee: MERCK PATENT GMBH
C12N5/0018C12M29/10C12N2500/33C12N2500/60
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Quick Facts
Patent No.
US 12709733
App. No.
17/776,336
Granted
Aug 18, 2026
Kind
B2
Abstract

The present invention relates to cell culture media comprising N-lactoyl derivatives of one or more amino acid. The poor solubility of some amino acids in cell culture media is overcome by substituting them with an N-lactoyl derivative.

Claims (21)

1 . A culture medium comprising at least one N-lactoyl-amino acid and/or salts thereof, wherein the culture medium is a chemically defined medium and is a dry powder medium, wherein the N-lactoyl-amino acid is selected from N-lactoyl-leucine, N-lactoyl-isoleucine, N-lactoyl-valine, N-lactoyl-phenylalanine, N-lactoyl-tyrosine and/or N-lactoyl-methionine.

2 . The culture medium according to claim 1 , characterized in that the cell culture medium comprises one or more of the components of formula I:

with R 1 + being H + or Na + , K + , Mg 2+ , Ca 2+ , Li +

and R 2 being the characteristic residue of the amino acid.

3 . The culture medium according to claim 1 , characterized in that the cell culture medium comprises the sodium salt of the one or more N-lactoyl amino acids.

4 . The culture medium according to claim 1 , characterized in that the cell culture medium comprises the one or more N-lactoyl amino acids and the corresponding amino acids and/or salts thereof.

5 . The culture medium according to claim 2 , characterized in that the medium comprises the one or more N-lactoyl amino acids and/or salts thereof but not the corresponding amino acids and/or salts thereof.

6 . A method for producing a cell culture medium according to claim 1 by

a) mixing one or more N-lactoyl-amino acids according to formula I with other components of the cell culture medium; and

b) subjecting the mixture of step a) to milling.

7 . A process for culturing prokaryotic or eukaryotic cells by

a) providing a bioreactor, and providing a cell culture medium according to claim 2 and dissolving said cell culture medium in water or an aqueous buffer;

b) mixing the cells to be cultured with the cell culture medium; and

c) incubating the mixture of step b).

8 . The process according to claim 7 , characterized in that the bioreactor is a perfusion bioreactor.

9 . A process for culturing prokaryotic or eukaryotic cells in a bioreactor by

(a) filling the cells into the bioreactor;

(b) incubating the cells in the bioreactor; and

(c) adding a medium to the bioreactor, continuously over the whole time or once or several times within the cells incubation time;

whereby the medium is prepared by providing a cell culture medium according to claim 1 and dissolving said cell culture medium in water or an aqueous buffer.

10 . The process according to claim 9 , characterized in that the N-lactoyl amino acid is N-lactoyl leucine and/or N-lactoyl isoleucine and/or salts thereof.