Devices, systems, and methods for automated dissection and processing of biological samples
Systems, apparatuses, and methods enable rapid, repeatable, and accurate dissection of arthropods. Arthropod dissection apparatuses includes a shear dissection mechanism having a primary shear body and a secondary shear body. The primary shear body includes at least an inlet channel, a first outlet channel, and a second outlet channel formed therein. The secondary shear body is disposed in an aperture of the primary shear body and has a dissection chamber formed therein. The secondary shear body is movable between a first position and a second position relative to the primary shear body, which causes a shearing action at a shearing interface between the secondary shear body and the primary shear body.
1 . An arthropod dissection apparatus, comprising:
a shear dissection mechanism, comprising:
a primary shear body having an inlet channel, a first outlet channel, and a second outlet channel formed therein; and
a secondary shear body disposed in an aperture of the primary shear body and having a dissection chamber formed therein, the secondary shear body being movable between a first position and a second position relative to the primary shear body, wherein in the first position, the dissection chamber pneumatically connects the inlet channel to the first outlet channel, wherein in the second position, the dissection chamber pneumatically connects the inlet channel to the second outlet channel,
wherein movement of the secondary shear body from the first position to the second position causes a shearing action at a shearing interface between the secondary shear body and the primary shear body;
the arthropod dissection apparatus further comprising a vacuum connector pneumatically couplable to the first outlet channel and the second outlet channel,
wherein the shear dissection mechanism is configured such that, when the vacuum connector is pneumatically coupled to a vacuum source and the secondary shear body is in the first position, the vacuum source draws a vacuum through the inlet channel, the dissection chamber, and the first outlet channel,
wherein the shear dissection mechanism is configured such that when the vacuum connector is pneumatically coupled to the vacuum source and the secondary shear body is in the second position, the vacuum source draws the vacuum through an air inlet of the secondary shear body, the dissection chamber, and the second outlet channel.
2 . The arthropod dissection apparatus of claim 1 , wherein the first outlet channel has a smaller diameter than the dissection chamber.
3 . The arthropod dissection apparatus of claim 2 , wherein the first outlet channel has a first diameter and wherein the dissection chamber has a second diameter, wherein the first diameter is 50%-75% of the second diameter.
4 . The arthropod dissection apparatus of claim 2 , wherein the first outlet channel has a first diameter and wherein the dissection chamber has a second diameter, wherein the first diameter is 0.50 mm-1.00 mm smaller than the second diameter.
5 . The arthropod dissection apparatus of claim 2 , wherein the first outlet channel has a smaller diameter than the second outlet channel.
6 . The arthropod dissection apparatus of claim 2 , wherein the first outlet channel and the dissection chamber form the shearing interface.
7 . The arthropod dissection apparatus of claim 1 , wherein the secondary shear body is a cylinder that rotates relative to the primary shear body between the first position and the second position.
8 . The arthropod dissection apparatus of claim 1 , further comprising:
a first specimen collection tube pneumatically coupled to the first outlet channel by a first pneumatic tubing, wherein the vacuum connector is pneumatically couplable to the first pneumatic tubing between the first specimen collection tube and the first outlet channel; and
a second specimen collection tube pneumatically coupled to the second outlet channel by a second pneumatic tubing, wherein the vacuum connector is pneumatically couplable to the second pneumatic tubing between the second specimen collection tube and the second outlet channel.
9 . The arthropod dissection apparatus of claim 8 , further comprising a pressure regulator pneumatically coupled between the first pneumatic tubing and the second pneumatic tubing.
10 . The arthropod dissection apparatus of claim 1 , further comprising a wand pneumatically coupled to the inlet channel of the shear dissection mechanism via a flexible pneumatic tubing.
11 . The arthropod dissection apparatus of claim 10 , wherein the flexible pneumatic tubing has a smaller diameter than the inlet channel, and wherein the inlet channel has a smaller diameter than the dissection chamber.
12 . The arthropod dissection apparatus of claim 1 , further comprising a drive operably coupled to move the secondary shear body between the first position and the second position.
13 . The arthropod dissection apparatus of claim 12 , further comprising an actuator and a controller operably coupled to the actuator and to the drive, wherein actuation of the actuator sends a signal to the controller, and in response to the signal, the controller sends a second signal to the drive, causing the drive to move the secondary shear body between the second position and the first position.
14 . The arthropod dissection apparatus of claim 13 , further comprising a display screen operably connected to the controller,
wherein the controller comprises logic, which when executed: stores a number of cycles of the actuator; and
displays the number of cycles on the display screen.
15 . An arthropod processing system, comprising:
the arthropod dissection apparatus of claim 1 ; and a grinder, comprising:
a pestle assembly comprising a rotating pestle; and
a carriage assembly comprising a carriage having a test tube aperture configured to hold a test tube, wherein the carriage is configured to translate relative to the rotating pestle.
16 . The arthropod processing system of claim 15 , wherein the carnage is configured to translate along a track relative to the pestle.
17 . The arthropod processing system of claim 16 , wherein the grinder further comprises a first drive operably coupled to a lead screw configured to translate the carriage along the track.
18 . The arthropod processing system of claim 17 , wherein the grinder further comprises a second drive configured to rotate the pestle.
19 . The arthropod processing system of claim 17 , wherein the grinder further comprises a second controller operably coupled to the pestle assembly and the carriage assembly,
wherein the second controller comprises logic, which when executed: advances the carriage toward the pestle; and
rotates the pestle a predetermined number of grinds based upon an input from a user input device.
20 . A method of dissecting an arthropod using the arthropod dissection apparatus of claim 1 , comprising:
moving the arthropod through the inlet channel of the primary shear body into the dissection chamber of the secondary shear body, wherein moving the arthropod comprises drawing the arthropod into the dissection chamber using the vacuum source pneumatically coupled to the vacuum connector that is pneumatically coupled to the first outlet channel of the primary shear body, the second outlet channel of the primary shear body, and the secondary shear body;
moving the arthropod partially, including by drawing the arthropod partially using the vacuum source, through a constriction between the dissection chamber and the first outlet channel connected to the dissection chamber such that a larger diameter portion of the arthropod is held in the dissection chamber by the constriction and a smaller diameter portion of the arthropod is disposed in the first outlet channel; and
shearing the larger diameter portion of the arthropod from the smaller diameter portion of the arthropod by moving the dissection chamber relative to the first outlet channel.
21 . The method of claim 20 , further comprising: evacuating the larger diameter portion of the arthropod through the second outlet channel.
22 . The method of claim 21 , further comprising: evacuating the smaller diameter portion of the arthropod through the first outlet channel.
23 . The method of claim 22 , wherein moving the dissection chamber relative to the first outlet channel includes rotating the dissection chamber relative to the first outlet channel.
24 . The method of claim 20 , further comprising grinding the larger diameter portion of the arthropod.