Nucleic acid extraction and fluorescent PCR detection system
A nucleic acid extraction and fluorescent PCR detection system, comprising: an extraction strip, an extraction strip loading device for loading the extraction strip, a sample holder loading device for loading and unloading samples, an extraction strip transfer gripper for driving the extraction strip to move to a preset position, a nucleic acid extraction device for purifying the samples to obtain nucleic acids, a PCR detection device for performing fluorescent PCR detection on the nucleic acids, and a control device, wherein the extraction strip loading device, the sample holder loading device, the extraction strip transfer gripper, the nucleic acid extraction device and the PCR detection device are all connected to the control device. The problems of relatively poor flexibility and relatively low detection efficiency of a nucleic acid extraction and detection system can be ameliorated, so that a sample detection process is more flexible and convenient.
1 . A nucleic acid extraction and fluorescent PCR test system, comprising: an extraction strip, an extraction-strip loading device which has a function of on-line adding the extraction strip and is configured to load the extraction strip, a sample-holder loading device which is configured to load and unload a sample, an extraction-strip transfer gripper which is configured to drive the extraction strip to move to a preset position, a nucleic-acid extraction device which is configured to refine the sample to obtain nucleic acid, a PCR test device which is configured to perform a fluorescent PCR test on the nucleic acid, and a control device;
wherein the extraction-strip loading device, the sample-holder loading device, the extraction-strip transfer gripper, the nucleic-acid extraction device and the PCR test device are connected to the control device,
wherein the PCR test device comprises a fluorescent PCR test device which is configured to perform fluorescent PCR tests on a sealed PCR tube; and
the fluorescent PCR test device is connected to the control device,
wherein the fluorescent PCR test device comprises: a housing, a photometry assembly and temperature control components, wherein the housing is provided with a plurality of test positions for placing the sealed PCR, and each of the plurality of test positions is provided with the corresponding temperature control component, so that the temperatures in the plurality of test positions are independently controlled to rise or fall; wherein the photometry assembly and the temperature control components are both connected to the control device; and
the photometry assembly comprises a light source assembly which is configured to provide a test light source and a driving component which is configured to drive the light source assembly to move; the number of the light source assembly is plural, the plurality of light source assemblies provide different types of light sources, and each light source assembly illuminates, driven by the driving component, the corresponding test position.
2 . The nucleic acid extraction and fluorescent PCR test system according to claim 1 , wherein the extraction-strip loading device comprises: a passage and a pushing device which is configured to push the extraction strip to move in the passage;
wherein one end of the passage is a gripping position for the extraction strip, the pushing device is movable transversely, and the pushing device is connected to the control device.
3 . The nucleic acid extraction and fluorescent PCR test system according to claim 2 , wherein the gripping position is provided with a jacking device which is configured to move up and down to separate the extraction strip, and the jacking device is connected to the control device.
4 . The nucleic acid extraction and fluorescent PCR test system according to claim 1 , wherein the sample-holder loading device comprises: a sample holder which is configured to load the sample, a loading pusher, an emergency pusher, a scanner, a transfer pusher and an unloading pusher, wherein the transfer pusher is configured to transfer the sample to a scanning position of the scanner and to move the sample after scanning to a sampling position, and the unloading pusher is configured to convey the sample after sampling to a sample recovery area; and
the loading pusher, the emergency pusher, the scanner, the transfer pusher, and the unloading pusher are all connected to the control device.
5 . The nucleic acid extraction and fluorescent PCR test system according to claim 1 , wherein the extraction-strip transfer gripper comprises: an openable gripper device which is configured to grip the extraction strip, a gripper lifting device which is configured to drive the gripper device to move up and down, and a gripper transverse-movement device which is configured to drive the gripper lifting device to move transversely, wherein the gripper device is arranged on the gripper lifting device, and the gripper lifting device is slidably connected with the gripper transverse-movement device; and
the gripper device, the gripper lifting device and the gripper transverse-movement device are all connected to the control device.
6 . The nucleic acid extraction and fluorescent PCR test system according to claim 1 , wherein the nucleic-acid extraction device comprises: a sealing-film piercing device which is configured to pierce through a sealing film of the extraction strip, a filling device which is configured to add the sample to be tested to the extraction strip, a reagent needle, a magnetic-bead washing device, a high-temperature dissociation device which is configured to perform high-temperature incubation on the sample, and a refined-solution magnetic-absorption device which is configured to perform magnetic absorption refinement on the sample to obtain a nucleic acid refined solution; and
the reagent needle is configured to add a corresponding reagent into the extraction strip or a PCR tube and to add the nucleic acid refined solution of the extraction strip into the PCR tube.
7 . The nucleic acid extraction and fluorescent PCR test system according to claim 6 , wherein the sealing-film piercing device comprises: a piercer and a driving assembly, wherein the driving assembly is connected with the piercer to drive the piercer to move; and when the piercer moves to a piercing position, the piercer pierces through the sealing film of the extraction strip; and the driving assembly is connected to the control device.
8 . The nucleic acid extraction and fluorescent PCR test system according to claim 6 , wherein the filling device comprises: a sample needle which is configured to draw the sample and a sample moving device which is configured to drive the sample needle to move in space, wherein the sample needle is connected to the sample moving device, and the sample moving device is connected to the control device.
9 . The nucleic acid extraction and fluorescent PCR test system according to claim 6 , wherein the high-temperature dissociation device comprises an incubation heating block which is configured to heat a reaction chamber of the extraction strip, and the incubation heating block is connected to the control device.
10 . The nucleic acid extraction and fluorescent PCR test system according to claim 6 , wherein the magnetic-bead washing device comprises: a detergent needle, a detergent driving device which is configured to drive the detergent needle to move in space, a magnet device which is configured to selectively apply a magnetic field to the reaction chamber of the extraction strip, and a magnet driving device which is configured to drive the magnet device to rotate;
wherein the magnet driving device is connected to the magnet device, and the detergent needle is connected to the detergent driving device; and
the magnet driving device and the detergent driving device are both connected to the control device.
11 . The nucleic acid extraction and fluorescent PCR test system according to claim 1 , further comprising a refrigeration-reagent supply device which has a refrigeration function and is configured to provide different reagents.
12 . The nucleic acid extraction and fluorescent PCR test system according to claim 1 , further comprising a discard device which is configured to recycle the discarded extraction strip, wherein the discard device is connected to the control device.