Analyzing device and analyzing method
View Patent ↗Provided is a device in which light from a sample 7 fixed in a test piece 1 is captured by an image sensor 5 through an optical system made up of a lens 3 , a diaphragm 4 , and so on and concentration information is obtained, wherein a wide-band light source 12 for illuminating the test piece 1 is combined with an optical filter 13 for optionally selecting a wavelength of the light captured by the image sensor. Thus it is possible to reduce a measurement error caused by a change of the light quantity distribution of the light source 12.
1. An analyzing method in which light from a sample set on a test piece is received by an image sensor having multiple pixels through an optical system having an optical filter and concentration information of the sample is obtained,
the analyzing method comprising:
a first step of calculating beforehand a luminance value obtained at any position in the image sensor based on dependence on a filter incident angle and wavelength characteristics of the light from the sample set with various known concentrations on the test piece;
a second step of calculating correction coefficients for matching luminance distributions obtained at the respective concentrations in the first step with a luminance distribution of any reference concentration selected from the concentrations;
a third step of making a rectilinear approximation by plotting the correction coefficients obtained at the respective concentrations in the second step, relative to the concentration information,
a fourth step of calculating a correction function of the dependence on a filter incident angle according to the reference concentration, based on the luminance distribution of the reference concentration;
a fifth step of correcting luminance obtained on the samples separately set to be measured with unknown concentrations on the test piece, by using the correction function obtained in the fourth step according to the reference concentration, and calculating the concentration information by using the corrected luminance;
a sixth step of obtaining concentration correction coefficients of the samples to be measured, by correlating the concentration information obtained in the fifth step with a straight line determined in the third step; and
a seventh step of integrating the concentration correction coefficients obtained in the sixth step to the correction function obtained in the fourth step according to the reference concentration, correcting the luminance of the samples to be measured, by using the correction functions after the integration, and obtaining again the concentration information of the samples to be measured, by using the corrected luminance.
2. An analyzing device in which light from a sample set on a test piece is received by an image sensor having multiple pixels through an optical system having an optical filter and concentration information of the sample is obtained,
the analyzing device comprising:
a luminance calculator for calculating a luminance value beforehand at any position in the image sensor based on dependence on a filter incident angle and wavelength characteristics of the light from the sample set with different known concentrations on the test piece;
a first correction coefficient calculator for calculating correction coefficients for matching luminance distributions obtained at the respective concentrations by the luminance calculator with a luminance distribution of any reference concentration selected from the concentrations;
a rectilinear approximation unit for making a rectilinear approximation by plotting the correction coefficients obtained at the respective concentrations by the first correction coefficient calculator, relative to the concentration information;
a correction function calculator for calculating a correction function of the dependence on the filter incident angle according to the reference concentration, based on the luminance distribution of the reference concentration;
a concentration calculator for correcting luminance obtained on the samples separately set to be measured with unknown concentrations on the test piece, by using the correction function obtained by the correction function calculator according to the reference concentration, and calculating the concentration information by using the corrected luminance;
a second correction coefficient obtaining unit for obtaining concentration correction coefficients of the samples to be measured, by correlating the concentration information obtained by the concentration calculator with a straight line determined by the rectilinear approximation unit; and
a concentration obtaining unit for integrating the concentration correction coefficients obtained by the second correction coefficient obtaining unit to the correction function obtained by the correction function calculator according to the reference concentration, correcting the luminance of the samples to be measured, by using the correction functions after the integration, and obtaining again the concentration information of the samples to be measured, by using the corrected luminance.