Granted Patent B1

US 7,939,317 · App. 07/839,194 · Granted May 10, 2011

Abstract

Claims (13)

1. A DNA construct comprising a gene encoding a protein, said gene being under transcriptional control of a mammalian milk protein promoter sequence which does not naturally control transcription of said gene, said DNA construct further comprising DNA encoding a peptide enabling secretion of said protein.

2. The DNA construct of claim 1 , wherein said secretion enabling peptide comprises a secretion signal peptide which is cleaved from said secretion protein.

3. The DNA construct of claim 1 , wherein said DNA encoding a peptide enabling secretion of said protein is the signal encoding sequence naturally associated with said gene encoding said protein.

4. The DNA construct of claim 1 , wherein said DNA encoding a peptide enabling secretion of said protein is the signal encoding sequence naturally associated with said mammalian milk protein promoter.

5. The DNA construct of claim 1 , wherein said DNA sequence includes a transcriptional stop sequence.

6. The DNA construct of claim 5 , wherein said stop sequence comprises the SV40 virus polyadenylation site.

7. The DNA construct of claim 1 , wherein said gene encodes human tissue plasminogen activator or hepatitis B surface antigen.

8. The DNA construct of claim 1 , wherein said milk protein is a milk serum protein.

9. The DNA construct of claim 8 , wherein said milk serum protein is alpha-lactalbumin.

10. The DNA construct of claim 1 , wherein the mammalian milk protein promoter sequence is the sequence of a milk serum protein promoter.

11. The DNA construct of claim 10 , wherein the milk serum protein promoter is an α-lactalbumin promoter.

12. The DNA construct of claim 10 , wherein the milk serum protein promoter is a β-lactoglobulin promoter.

13. The DNA construct of claim 1 , wherein the mammalian milk protein promoter sequence is the sequence of a casein protein promoter.

Assignments (8)