Glial mitogenic factors, their preparation and use
View Patent ↗Disclosed are methods for inducing myelination of neural cells by glial cells, The methods involve contacting glial cells with polypeptides comprising epidermal growth factor-like domains encoded by the GGF/p185 erb B2 ligand gene provided as a feature of the invention.
1. A method for inducing myelination of a neural cell by a glial cell, said method comprising contacting said glial cell with an amount of a polypeptide which comprises an epidermal growth factor-like domain, wherein said epidermal growth factor-like domain comprises an amino acid sequence which is identical to an amino acid sequence encoded by a GGF/p185 B2 ligand gene, and wherein said amino acid sequence comprises an amino acid sequence encoded by a nucleic acid sequence selected from the group consisting of:
SEQ ID NO: 154 (EGFL1);
SEQ ID NO: 155 (EGFL2);
SEQ ID NO: 156 (EGFL3);
SEQ ID NO: 157 (EGFL4);
SEQ ID NO: 158 (EFGL5);
SEQ ID NO: 159 (EFGL6); and
amino acids 53-103 encoded by SEQ ID NO: 150.
2. A method for inducing myelination of a neural cell by a glial cell, said method comprising contacting said glial cell with an amount of a polypeptide which comprises an epidermal growth factor-like domain, wherein said epidermal growth factor-like domain comprises the amino acid sequence set forth in SEQ ID NO: 188.
3. A method for inducing myelination of a neural cell by a glial cell, said method comprising contacting said glial cell with an amount of a polypeptide which comprises an epidermal growth factor-like domain, wherein said epidermal growth factor-like domain comprises the amino acid sequence set forth in SEQ ID NO: 189.
4. A method for inducing myelination of a neural cell by a glial cell, said method comprising contacting said glial cell with an amount of a polypeptide which comprises an epidermal growth factor-like domain, wherein said epidermal growth factor-like domain comprises an amino acid sequence which is identical to an amino acid sequence encoded by a GGF/p185 erb B2 ligand gene, and wherein said amino acid sequence is selected from the group consisting of:
SEQ ID NO: 1.51;
SEQ ID NO: 152; and
amino acids 362-411 SEQ ID NO: 170
wherein said contacting with said polypeptide is sufficient to induce myelination of said neural cell by said glial cell.
5. The method of claim 4 , wherein said amino acid sequence is SEQ ID NO: 151.
6. The method of claim 4 , wherein said amino acid sequence is SEQ ID NO: 152.
7. The method of claim 1 , 2 , 3 , or 4 , wherein said polypeptide is a recombinant polypeptide with glial cell mitogenic activity.