Tiling process for constructing a chemical array
View Patent ↗A process of constructing an array of chemical moieties having the following steps: forming multiple discrete physical entities (tiles) from a substantially planar material having one or more species of chemical moiety attached thereto; and picking and placing the entities (tiles) stably on a support at spatially distinct ascertainable locations to form an array of chemical moieties. The formed array includes at least two species of chemical moiety and preferably from about 50 to about 1000 species. The claimed invention includes an array formed by this process.
1. A process for constructing an chemical array comprising a plurality of species of bioorganic molecules in a predetermined arrangement, said process comprising the steps of:
1) for each species of said plurality of bioorganic molecules, constructing a batch of separate tiles by:
(a) providing a unit of a substantially planar solid material having an attachment surface;
(b) attaching said species of bioorganic molecule onto said attachment surface; and
(c) subdividing said unit of substantially planar material to form a plurality of separate tiles, a surface of each of said separate tiles comprising a portion of said attachment surface; and
2) affixing separate tiles from the said batches of tiles in predetermined spatial positions on a support.
2. The process of claim 1 wherein said affixing step 2 comprises affixing said tile to said support by insertion into a pocket formed on said support.
3. The process of claim 1 wherein said affixing step (2) comprises affixing said tile to said support with an adhesive.
4. The process of claim 1 wherein said attaching step (b) comprises contacting said attachment surface of said material with a solution of said species of bioorganic molecule.
5. The process of claim 1 wherein said species of bioorganic molecule is selected from the group consisting of nucleic acids, proteins, polysaccharides and lipids.
6. The process of claim 1 wherein said array comprises from about 50 to about 1000 different species of said bioorganic molecules and wherein said bioorganic molecules are attached to said surface of each said tile at a density of about 1000 to 100,000 bioorganic molecules per square micron of said attachment surface.
7. The process of claim 1 wherein said species of bioorganic molecule is a nucleic acid or a protein.
8. The process of claim 1 wherein said subdividing step (c) is carried out prior to said attaching step (b).
9. The process of claim 1 additionally comprising:
prior to said subdividing step (c), said unit of substantially planar material is affixed onto a backing material, whereby said separate tiles remain affixed in place on said backing material following said subdividing step (c); and
removing the separate tiles from the backing material prior to affixing the tiles on the support.
10. The process of claim 9 wherein said backing material comprises an adhesive film.
11. The process of claim 1 , further comprising, prior to said attaching step (b), a step of derivatizing said attachment surface.
12. The process of claim 1 wherein said material comprises a solid nonporous material selected from the group consisting of a glass, a silicon, and a plastic.
13. The process of claim 1 wherein said separate tiles are regularly-shaped and said surface of each said separate tile has a longest dimension from about 100 μm to about 1 mm.
14. The process of claim 1 wherein said separate tiles are regularly-shaped and said surface of each said separate tile has a longest dimension from about 250 μm to about 1 mm.
15. The process of claim 1 wherein, for each said unit of material having an attachment surface, at least one additional species of bioorganic molecule is attached onto said attachment surface.
16. The process of claim 1 wherein, for each said unit of material having an attachment surface, at least one additional species of bioorganic molecule is attached onto said attachment surface.
17. The process of claim 1 additionally comprising bringing the constructed array into contact with a same sample.
18. The process of claim 1 additionally comprising performing a quality test on the attachment surface after the attaching and prior to the subdividing.
19. The process of claim 1 additionally comprising verifying the fidelity of the bioorganic molecules on the attachment surface prior to affixing tiles from the batches onto the support.
20. The process of claim 1 additionally comprising verifying the density of attachment of the bioorganic molecules on the attachment surface prior to affixing tiles from the batches onto the support.
21. A process according to claim 1 wherein the subdividing step (c) is carried out after said attaching step (b).
22. A process for constructing a chemical array comprising a plurality of species of nucleic acids in a predetermined arrangement, said process comprising the steps of:
1) for each species of said plurality of nucleic acids, constructing a batch of separate tiles by:
(a) providing a unit of a substantially planar solid material having an attachment surface;
(b) providing said species of nucleic acid onto said attachment surface so that they are attached thereto; and
(c) subdividing said unit of substantially planar material to form a plurality of separate tiles, a surface of each of said separate tiles comprising a portion of said attachment surface; and
2) affixing separate tiles from the said batches of tiles in predetermined spatial positions on a support.
23. A process according to claim 22 wherein the nucleic acids are oligonucleotides.
24. A process according to claim 22 wherein in step (b) the nucleic acid is presynthesized before attachment onto said surface.
25. A process according to claim 22 wherein the subdividing step (c) is carried out after said attaching step (b).
26. A process for constructing a chemical array comprising a plurality of species of proteins in a predetermined arrangement, said process comprising the steps of:
1) for each species of said plurality of proteins, constructing a batch of separate tiles by:
(a) providing a unit of a substantially planar solid material having an attachment surface;
(b) providing said species of proteins onto said attachment surface so that they are attached thereto; and
(c) subdividing said unit of substantially planar material to form a plurality of separate tiles, a surface of each of said separate tiles comprising a portion of said attachment surface; and
2) affixing separate tiles from the said batches of tiles in predetermined spatial positions on a support.
27. A process according to claim 26 wherein in step (b) the protein is presynthesized before attachment onto said surface.
28. A process for constructing a chemical array comprising a plurality of species of peptides in a predetermined arrangement, said process comprising the steps of:
1) for each species of said peptides, constructing a batch of separate tiles by:
(a) providing a unit of a substantially planar solid material having an attachment surface;
(b) providing said species of peptide onto said attachment surface so that they are attached thereto; and
(c) subdividing said unit of substantially planar material to form a plurality of separate tiles, a surface of each of said separate tiles comprising a portion of said attachment surface; and
2) affixing separate tiles from the said batches of tiles in predetermined spatial positions on a support.
29. A process according to claim 28 wherein in step (b) the peptide is presynthesized before attachment onto said surface.
30. A process according to claim 26 wherein the subdividing step (c) is carried out after said attaching step (b).
31. A process according to claim 28 wherein the subdividing step (c) is carried out after said attaching step (b).