DNA encoding human papilloma virus type 18
View Patent ↗The present invention is directed to DNA molecules encoding purified human papillomavirus type 18 and derivatives thereof.
1. An essentially purified HPV18 L1 protein comprising a sequence of amino acids as set forth in SEQ ID NO:2.
2. Virus-like particles comprised of recombinant L1 protein, or recombinant L1+L2 proteins of human papillomavirus 18, wherein the L1 protein comprises a sequence of amino acids as set forth in SEQ ID NO:2 and the L2 protein comprises a sequence of amino acids as set forth in SEQ ID NO:4, wherein the recombinant L1 protein or the recombinant L1+L2 proteins are produced in yeast.
3. A method of producing the virus-like particles of claim 2 , comprising:
(a) transforming yeast with a recombinant DNA molecule encoding papillomavirus L1 protein or papillomavirus L1+L2 proteins; wherein the L1 protein comprises a sequence of amino acids as set forth in SEQ ID NO:2 and the L2 protein comprises a sequence of amino acids as set forth in SEQ ID NO:4
(b) cultivating the transformed yeast under conditions that permit expression of the recombinant DNA molecule to produce the recombinant papillomavirus protein; and
(c) isolating the recombinant papillomavirus protein to produce the virus-like particles of claim 2 .
4. Recombinant papillomavirus protein produced by the method of claim 3 .
5. A vaccine comprising the virus-like particles of claim 2 and a pharmaceutically acceptable carrier.
6. Pharmaceutical compositions comprising the virus-like particles of claim 2 and a pharmaceutically acceptable carrier.
7. A method of preventing papillomavirus infection comprising administering the vaccine of claim 5 to a host.
8. A method for inducing an immune response in an animal comprising administering the virus-like particles of claim 2 to an animal.