Method for identifying validated target and assay combination for drug development
View Patent ↗The invention comprises methods useful within a larger process for identifying compounds and/or designing further compounds with activity to produce a desired phenotype (for example, growth inhibition) in cells whose target cell component is the subject of certain studies to identify such compounds. The invention employs constructed cells comprising a regulable gene encoding a biomolecule which modulates (inhibits or activates) in vivo the function of a target component of the cell which can be an enzyme for example. The process incorporates methods for identifying biomolecules that bind to a chosen target cell component in vitro, methods for identifying biomolecules that also bind to the chosen target and modulate its function intracellularly, causing a phenotypic effect. The intracellular effect of a biomolecule can be tested in cell culture, or tested after introduction of the constructed cells into a host mammal in vivo, and methods for identifying compounds that compete with the biomolecules for sites on the target in competitive binding assays. Compounds identified by the series of steps in this process are candidates for drugs with the desired activity on the cell. Targets for which such compounds can be identified are validated as being essential to a phenotype of the cell.
1. A method for determining whether a biomolecule inhibits growth of cells in vivo, comprising:
a) introducing a cell having an exogenous regulable gene encoding a biomolecule into one or more test animals and into one or more control animals, wherein the biomolecule binds a protein target component of the cells;
b) regulating expression of the exogenous gene in the test animals to allow production of the biomolecule; and
c) monitoring the test animals for growth of the cells wherein observing fewer of the cells or a slower growth rate of the cells in the test animals compared to the number of the cells or growth rate of the cells in the control animals indicates that the biomolecule inhibits growth of cells in vivo.
2. The method of clam 1 , further comprising the step of first constructing the cell having the exogenous regulable gene encoding the biomolecule.
3. The method of claim 1 , further comprising the steps of first
i) constructing the cell having the exogenous regulable gene encoding the biomolecule;
ii) regulating expression of the exogenous regulable gene in a culture of constructed cells, thereby producing the biomolecule in the constructed cells; and
iii) monitoring growth of the constructed cells in culture, relative to growth of control cells, whereby, if growth is decreased in the constructed cell, compared to growth of the control cell, then the biomolecule inhibits growth in culture.
4. The method of claim 1 , wherein the protein target component comprises a complex comprising more than one gene product.
5. The method of claim 1 , wherein the protein target component consists of a single gene product.
6. The method of claim 1 , wherein the protein target component comprises an enzyme.
7. The method of claim 1 , wherein the biomolecule is a polypeptide or a peptide.
8. The method of claim 1 , wherein the biomolecule is a fusion protein.
9. The method of claim 1 , wherein the cell is a mammalian cell.
10. The method of claim 1 , wherein the cell is a pathogen cell.
11. The method of claim 2 , wherein the protein target component consists of a single gene product, a complex comprising more than one gene product, or an enzyme.
12. The method of claim 11 , wherein the protein target component comprises an enzyme.
13. The method of claim 12 , wherein the biomolecule is a polypeptide.
14. The method of claim 12 , wherein the biomolecule is a peptide.
15. The method of claim 12 , wherein the biomolecule is a fusion protein.
16. The method of claim 11 , wherein the cell is a mammalian cell.
17. The method of claim 11 , wherein the cell is a pathogen cell.
18. A method for determining whether a biomolecule inhibits infection by a pathogen cell in vivo, comprising
a) introducing a pathogen cell having an exogenous regulable gene encoding a biomolecule into one or more test animals and into one or more control animals, wherein the biomolecule binds a protein target component of the pathogen cell;
b) regulating expression of the exogenous gene in the test animals to allow production of the biomolecule; and
c) monitoring the test animals for signs of infection wherein observing fewer or less severe signs of infection in the test animal compared to signs of infection in the control animal indicates that the biomolecule inhibits infection by the pathogen in vivo.
19. The method of claim 18 , further comprising the step of first constructing the pathogen cell having the exogenous regulable gene encoding the biomolecule.
20. The method of claim 18 , further comprising the steps of first
i) constructing the pathogen cell having the exogenous regulable gene encoding the biomolecule;
ii) regulating expression of the exogenous regulable gene in a culture of constructed pathogen cells, thereby producing the biomolecule in the constructed pathogen cells; and
iii) monitoring growth of the constructed pathogen cells in culture, relative to growth of control cells, whereby, if growth is decreased in the constructed pathogen cell, compared to growth of the control cell, then the biomolecule inhibits growth in culture.
21. The method of claim 18 , wherein the protein target component comprises a complex comprising more than one gene product.
22. The method of claim 18 , wherein the protein target component consists of a single gene product.
23. The method of claim 18 , wherein the protein target component comprises an enzyme.
24. The method of claim 18 , wherein the biomolecule is a peptide or a polypeptide.
25. The method of claim 18 , wherein the biomolecule is a fusion protein.
26. The method of claim 19 , where the protein target component consists of a single gene product, a complex comprising more than one gene product, or an enzyme.
27. The method of claim 26 , wherein the protein target component comprises an enzyme.
28. The method of claim 27 , wherein the biomolecule is a polypeptide.
29. The method of claim 27 , wherein the biomolecule is a peptide.
30. The method of claim 27 , wherein the biomolecule is a fusion protein.
31. A method for identifying a compound which is a candidate for producing a phenotypic effect in a cell in vivo, said method comprising the steps of:
a) introducing a cell having an exogenous regulable gene encoding a biomolecule into one or more animals;
b) regulating expression of the exogenous gene in the animals to allow production of the biomolecule;
c) monitoring said cell in the animal for the phenotypic effect; and
d) identifying, if the biomolecule caused the phenotypic effect, one or more compounds that competitively bind to a target cell component to which the biomolecule binds, whereby if the compound competitively binds to the target cell component, then the compound is a candidate for producing the phenotypic effect in vivo.
32. The method of claim 31 , further comprising the step of first constructing the cell having the exogenous regulable gene encoding the biomolecule.
33. The method of claim 31 , further comprising the steps of first
i) constructing the cell having the exogenous regulable gene encoding the biomolecule;
ii) regulating expression of the exogenous regulable gene in a culture of constructed cells, thereby producing the biomolecule in the constructed cells; and
ii.) monitoring growth of the constructed cells in culture, relative to growth of control cells, whereby, if growth is decreased in the constricted cell, compared to growth of the control cell, then the biomolecule inhibits growth in culture.
34. The method of claim 31 , wherein the biomolecule is a polypeptide or a peptide.
35. The method of claim of claim 31 , wherein the biomolecule is a fusion protein.
36. The method of claim 31 , wherein the cell is a mammalian cell.
37. The method of claim 31 , wherein the cell is a pathogen cell.
38. The method of claim 31 wherein the phenotypic effect is growth inhibition.
39. The method of claim 32 wherein the phenotypic effect is growth inhibition.
40. The method of claim 39 wherein the biomolecule is a peptide or polypeptide.
41. The method of claim 40 , wherein the biomolecule is a fusion protein.
42. The method of claim 41 wherein the cell is a mammalian cell.
43. The method of claim 41 wherein the cell is a pathogen cell.