The present invention relates to nucleotide sequences, including expressed sequence tags (ESTs), oligonucleotide probes, polypeptides, antagonists and agonists vectors and host cells expressing, and immunoadhesions and antibodies to PRO201, PRO308 or PRO309 polypeptides. The invention further relates to compositions and method for the diagnosis and treatment of neoplastic cell growth and proliferation in mammals, including humans. The invention is based in part on the identification of genes that are amplified in the genome of tumor cells. Such gene amplification is expected to be associated with the overexpression of the gene product and contribute to tumorigenesis. Accordingly, the proteins encoded by the amplified genes are believed to be useful targents for the diagnosis and/or treatment (including prevention) of certain tumors (e.g., cancer) and may act as predictors of the prognosis of tumor treatment.
1. An antibody which specifically binds to a polypeptide comprising the amino acid sequence of SEQ ID NO:1.
2. An antibody which specifically binds to a polypeptide comprising the amino acid sequence of SEQ ID NO:3.
3. An antibody which specifically binds to a polypeptide comprising the amino acid sequence of SEQ ID NO:5.
4. The antibody of any one of claims 1 to 3 , wherein said antibody is a monoclonal antibody.
5. The antibody of claim 1 which induces the death of a cell expressing said polypeptide comprising said amino acid sequence of SEQ ID NO:1.
6. The antibody of claim 2 which induces the death of a cell expressing said polypeptide comprising said amino acid sequence of SEQ ID NO:3.
7. The antibody of claim 3 which induces the death of a cell expressing said polypeptide comprising said amino acid sequence of SEQ ID NO:5.
8. The antibody of any one of claims 5 to 7 , wherein said cell is a tumor cell.
9. The antibody of claim 4 which is labelled.
10. The antibody of claim 4 which is immobilized on a solid support.
11. The antibody of claim 4 which is an antigen binding fragment or a single-chain antibody.
12. A composition comprising an antibody of claim 1 in admixture with a pharmaceutically acceptable carrier.
13. A composition comprising an antibody of claim 2 in admixture with a pharmaceutically acceptable carrier.
14. A composition comprising an antibody of claim 3 in admixture with a pharmaceutically acceptable carrier.
15. A cancer diagnostic kit comprising the antibody of any one of claims 1 to 3 and a carrier in suitable packaging.
16. The antibody of claim 4 which is bispecific.
17. The antibody of any one of claims 1 to 3 , wherein the portion binding said polypeptide is an F(ab′) 2 fragment.
18. The antibody of claim 4 which is heteroconjugated.
19. The antibody of claim 4 which is formulated as an immunoliposome.
20. The antibody of claim 11 , wherein the antigen binding fragment is selected from the group consisting of: Fab, Fab′, F(ab′) 2 and Fv.
21. The antibody of claim 20 which is an Fv fragment.
22. The antibody of claim 20 which is an Fab fragment.
23. The antibody of claim 20 which is an Fab′ fragment.