Method for inducing a cell-mediated immune response and improved parenteral vaccine formulations thereof
View Patent ↗A method of inducing either a T H 1 polarized immune response, a T H 2 polarized immune response or a combined T H 1 and T H 2 response to an antigen and associated vaccine formulations are disclosed. A method is provided for inducing a polarized T H 1 response by parenteral administration of microparticles sized such that at least 50% of the microparticles are less than 5 μm. The microparticles containing antigen entrapped or encapsulated by a biodegradable polymer. Additionally, a method is provided for inducing a polarized T H 2 response by parenteral administration of nanoparticles sized such that at least 50% of the nanoparticles are less than 600 nm, the nanoparticles containing antigen entrapped or encapsulated by a biodegradable polymer. Vaccine formulations containing the B. pertussis antigens PTd, FHA or a combination of PTd and FHA are provided.
1. A method of inducing a T H 1 polarized immune response to at least one antigen comprising parenterally administering to a subject microparticles comprising said at least one antigen entrapped or encapsulated in a biodegradable polymer, wherein said biodegradable polymer comprises a copolymer of lactic acid and glycolic acid or enantiomers thereof, and wherein said microparticles are sized such that the average diameter of said microparticles is from 2.4 μm to 4.3 μm and at least 50% of the microparticles are less than 5 μm.
2. The method of claim 1 , wherein the microparticles are sized such that at least 50% of the microparticles are less than 3 μm.
3. The method of claim 1 , wherein the microparticles are formed using a solvent evaporation method.
4. The method of claim 1 , wherein the at least one antigen comprises a B. pertussis antigen.
5. The method of claim 1 , wherein the parenteral administration is selected from the group consisting of intraperitoneal administration, subcutaneous administration and intramuscular administration.
6. A vaccine formulation for enhancing a T H 1 immune response to at least one antigen and adapted for parenteral administration comprising a pharmaceutically acceptable carrier and a pharmaceutically effective amount of microparticles comprising said at least one antigen entrapped or encapsulated in a biodegradable polymer, wherein said biodegradable polymer comprises a copolymer of lactic acid and glycolic acid or enantiomers thereof, and wherein said microparticles are sized such that average diameter of said microparticles is from 2.4 μm to 4.3 μm and at least 50% of the microparticles are less than 5 μm.
7. The vaccine formulation of claim 6 , wherein the microparticles are sized such that at least 50% of the microparticles are less than 3 μm.
8. The vaccine formulation of claim 6 , wherein the microparticles are formed using a solvent evaporation method.
9. The vaccine formulation of claim 6 , wherein the at least one antigen comprises a B. pertussis antigen.
10. A vaccine formulation for enhancing a T H 1 immune response to at least one antigen and adapted for parenteral administration comprising a pharmaceutically acceptable carrier and a pharmaceutically effective amount of microparticles comprising at least 2 subpopulations of microparticles, each subpopulation comprising a different antigen, each antigen entrapped or encapsulated by a biodegradable polymer, comprises a copolymer of lactic acid and glycolic acid of enantiomers thereof, and wherein said microparticles are sized such that the average diameter of said microparticles is from 2.4 μm to 4.3 μm and at least 50% of the microparticles are less than 5 μm.