Low temperature adaptor for evaporative light detection
View Patent ↗The present invention is directed to a system for converting between single flow and split flow evaporative light scattering detection devices for detecting samples in a mobile phase. Included in the system is a evaporative light scattering detection device, a low temperature adaptor, and a connection tube for providing a fluid connection between the evaporative light scattering detection device and the low temperature adaptor.
1. Detecting consecutive samples by evaporative light scattering detection according to the split-flow method and the single-flow method of evaporative scattering light detection comprising the steps of:
(a) providing an evaporative light scattering device comprising a nebulizer; a drift tube channel downstream in the direction of sample flow and in flow communication with the nebulizer, a photodetector downstream in the direction of sample flow from the nebulizer; and a light source downstream in the direction of sample flow from the nebulizer;
(b) flowing a first sample comprising previously separated components to be detected and mobile phase through the evaporative light scattering device and detecting the separated components of the, first sample by one of the evaporative light scattering detection methods selected from the group consisting of the split-flow method and the single-flow method;
(c) after step (b), flowing a second sample comprising previously separated components to be detected, and mobile phase through the evaporative light scattering device and detecting the separated components of the, second sample by the evaporative light scattering detection method selected from the group consisting of the split-flow method and the single-flow method wherein the method selected in step (c) is different than the method selected in step (b).
2. The method of claim 1 wherein the temperature in the drift tube channel is controlled by heat tape affixed to the drift tube.
3. The method of claim 1 wherein the method selected in step (b) is the split-flow method and the evaporative light scattering device used in step (b) further comprises a low temperature adaptor positioned downstream of the nebulizer and upstream of the photodetector in the direction of sample flow through the device.
4. The method of claim 3 wherein the low temperature adaptor comprises a coil and nebulization chamber.
5. The method of claim 3 wherein the temperature in the drift tube channel is controlled by heat tape affixed to the drift tube.
6. The method of claim 4 wherein the low temperature adaptor further comprises a sweep gas channel.
7. The method of claim 5 wherein the temperature in the nebulization chamber and coil is controlled by heat tape affixed at predetermined intervals to the nebulization chamber and coil.