Non-toxic non-toxigenic non-pathogenic fusarium expression system
View Patent ↗The present invention relates non-toxic, non-toxigenic, non-pathogenic recombinant Fusarium host cells of the section Discolor or a teleomorph or synonym thereof, comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter.
1. A method for producing a heterologous protein comprising: (a) culturing a non-toxic, non-toxigenic, and non-pathogenic Fusarium venenatum host cell comprising a nucleic acid sequence operably linked to a promoter encoding the heterologous protein, wherein the non-toxic, non-toxigenic, and non-pathogenic Fusarium venenatum host cell has the morphological and growth characteristics and the non-toxic, non-toxigenic, and non-pathogenic properties of Fusarium venenatum deposited under NRRL 30747; and (b) isolating the heterologous protein.
2. An isolated non-toxic, non-toxigenic, and non-pathogenic Fusarium venenatum host cell comprising a nucleic acid sequence encoding a heterologous protein, wherein the non-toxic, non-toxigenic, and non-pathogenic Fusarium venenatum host cell has the morphological and growth characteristics and the non-toxic, non-toxigenic, and non-pathogenic properties of Fusarium venenatum deposited under NRRL 30747.
3. The method of claim 1 , wherein the host cell is the non-toxic, non-toxigenic, and non-pathogenic Fusarium venenatum host cell deposited under NRRL 30747.
4. The Fusarium venenatum host cell of claim 2 , which is the non-toxic, non-toxigenic, and non-pathogenic Fusarium venenatum host cell deposited under NRRL 30747.