Topical use of probiotic
View Patent ↗Compositions including an isolated Bacillus species, spores or an extracellular product of B. coagulans , suitable for topical application, for inhibiting growth of yeast, fungus, bacteria or Herpes simplex virus are disclosed. Methods of inhibiting growth of yeast, fungus, bacteria or Herpes simplex virus by topical application of compositions that include an isolated Bacillus species, spores or an extracellular product of a B. coagulans strain are disclosed.
1. A method of inhibiting yeast or fungal infection comprising:
applying topically to skin or a mucous membrane of a mammal a probiotic composition comprising a bacterial component, said bacterial component consisting of Bacillus coagulans Hammer (ATCC# 31284); and allowing the Bacillus coagulans bacteria to grow topically for sufficient time to inhibit growth of yeast or fungus, thereby inhibiting the yeast or fungal infection.
2. The method of claim 1 , wherein said Bacillus coagulans bacteria are provided in the form of spores.
3. The method of claim 1 wherein said composition contains 10 3 to 10 12 viable bacteria or spores per gram of composition.
4. The method of claim 1 wherein said method comprises applying from 10 8 to 10 10 viable bacteria or spores per day.
5. The method of claim 1 wherein said method comprises applying from 5×10 8 to 10 9 viable bacteria or spores per day.
6. The method of claim 1 wherein said composition comprises an effective amount of a fructo-oligosaccharide (FOS).
7. The method of claim 6 wherein said FOS is present in an amount of from about 10 to 1000 milligrams per gram of composition.
8. The method of claim 6 wherein said FOS is present in an amount of from about 100 to 500 milligrams per gram of composition.
9. The method of claim 1 , wherein said fungus is selected from the group consisting of Trichophyton mentagrophytes, Trichophyton interdigitale, Trichophyton rubrum , and Trichophyton yaoundei.
10. The method of claim 1 , wherein the applying step comprises applying a probiotic composition in the form of a cream, lotion, gel, oil, ointment, suspension, aerosol spray, powder, aerosol powder or semi-solid formulation.
11. A method of inhibiting growth of yeast or fungus or a combination thereof, comprising: applying a composition comprising a bacterial component consisting of Bacillus coagulans Hammer (ATCC# 31284) to a solid surface; contacting the solid surface with the applied Bacillus coagulans bacteria thereon to skin or a mucous membrane of a mammal; and allowing the solid surface to contact the skin or mucous membrane for sufficient time to allow initiation of probiotic activity of the Bacillus coagulans bacteria to inhibit growth of yeast or fungus or a combination thereof adjacent to or on the skin or mucous membrane.
12. The method of claim 11 , wherein the solid surface comprises a flexible article selected from the group consisting of a diaper, pliable material for wiping skin or a mucous membrane, dermal patch, adhesive tape, absorbent pad, tampon and article of clothing.
13. The method of claim 11 , wherein the applying step comprises impregnating the composition into a fibrous or nonfibrous solid matrix.
14. The method of claim 11 , wherein the bacterial component is included in the composition in the form of spores.
15. The method of claim 11 , wherein the bacterial component is included in the composition in the form of a dried cell mass.
16. The method of claim 11 wherein said composition contains 10 3 to 10 12 viable bacteria or spores per gram of composition.
17. The method of claim 11 wherein said composition comprises an effective amount of a fructo-oligosaccharide (FOS).
18. The method of claim 17 wherein said FOS is present in an amount of from about 10 to 1000 milligrams per gram of composition.
19. The method of claim 17 wherein said FOS is present in an amount of from about 100 to 500 milligrams per gram of composition.
20. A method of treating a vaginal infection, comprising: identifying a subject suffering from a vaginal infection; and applying topically to the skin or a mucous membrane of said subject a composition comprising a bacterial component consisting of Bacillus coagulans Hammer (ATCC# 31284).
21. The method of claim 20 , wherein said infection is caused by a yeast pathogen.
22. The method of claim 21 , wherein said yeast pathogen is selected from the group consisting of Candida albicans, Candida tropicalis , and a combination thereof.
23. The method of claim 20 , wherein said composition is in a form selected from the group consisting of a douche, bath salt, soap, powdered bubble bath, bath powder, bath oil, cream, liquid, powder, non-soap emollient cleanser, suppository, soft towelette, and aerosol microparticulate.
24. The method of claim 20 , wherein the Bacillus coagulans bacteria are in the form of spores.
25. The method of claim 20 , wherein the Bacillus coagulans bacteria are in the form of vegetative cells.
26. The method of claim 20 , wherein said composition contains 10 3 to 10 12 viable bacteria or spores per gram of composition.
27. The method of claim 23 , wherein said bath oil further comprises mineral oil, laureth-4, quaternium-18 hectorite and phenylcarbinol.
28. The method of claim 23 , wherein said bath oil further comprises olive oil, grape seed oil, emu oil, sweet almond oil, geranium oil, grapefruit oil, mandarin oil or peppermint oil.
29. The method of claim 23 , wherein said bath oil further comprises a fragrance.
30. The method of claim 23 , wherein said non-soap emollient cleanser further comprises sodium octoxynol-2 ethane sulfonate, petrolatum, octoxynol-3, mineral oil, lanolin oil, cocamide MEA, or imidazolidinyl urea.
31. The method of claim 23 , wherein said soft towelette further comprises potassium sorbate and disodium EDTA.
32. The method of claim 23 , wherein said soft towelette further comprises DMDM hydantoin, isopropyl myristate, methylparaben, polysorbate 60, propylene glycol, propylparaben or sorbitan stearate.
33. The method of claim 23 , wherein said soft towelette is disposable.
34. The method of claim 23 , wherein a symptom of said vaginal infection is selected from the group consisting of vaginal itch and discharge.
35. The method of claim 20 , wherein said composition is in the form of a vaginal suppository or insert comprising from about 10 6 to 10 12 viable Bacillus coagulans bacteria.
36. The method of claim 35 , wherein between one and about three suppositories or inserts are used per day for a consecutive period of time of about three to about seven days.
37. The method of claim 1 , wherein said yeast pathogen is selected from the group consisting of Candida albicans, Candida tropicalis , and a combination thereof.
38. The method of claim 11 , wherein said yeast pathogen is selected from the group consisting of Candida albicans, Candida tropicalis , and a combination thereof.