Antigen protein and nucleic acid coding for said protein
View Patent ↗To provide a novel antigenic protein and a nucleic acid encoding the antigenic protein, which are useful for prophylaxis, treatment and diagnosis of diseases caused by fungi including Candida albicans . An antigenic protein characterized in that the antigenic protein is recognized by antiserum derived from a mammal having Candida albicans -infection resistance; and a nucleic acid encoding an antigenic protein which is recognized by antiserum derived from a mammal having Candida albicans -infection resistance.
1. An isolated antigenic polypeptide which is recognized by an antiserum from a mammal having resistance to Candida albicans infection, wherein said antigenic polypeptide is:
(A) a polypeptide having the amino acid sequence of SEQ ID NO: 1;
(B) a polypeptide encoded by a nucleic acid having the nucleotide sequence of SEQ ID NO: 10; or
(C) a polypeptide encoded by a nucleic acid capable of hybridizing with a nucleic acid which encodes a polypeptide having the amino acid sequence of SEQ ID NO: 1, or a nucleic acid having the nucleotide sequence of SEQ ID NO: 10, or a nucleic acid fully complementary thereto, under stringent conditions which include incubation at 50° C. for 12 to 20 hours together with a nucleic acid probe in 6×SSC, wherein 1×SSC has 0.15M NaCl, 0.015M sodium citrate and a pH of 7.0 and contains 0.5% by weight of SDS, 0.1% by weight of bovine serum albumin, 0.1% by weight polyvinyl pyrrolidone, 0.1% by weight of a synthetic, water soluble, nonionic copolymer of sucrose and epichlorhydrin having a molecular weight of about 400,000, and 0.01% by weight of denatured salmon sperm DNA.
2. An isolated nucleic acid encoding an antigenic polypeptide which is recognized by an antiserum from a mammal having resistance to Candida albicans infection, wherein said nucleic acid is:
(A) a nucleic acid encoding polypeptide having the amino acid sequence of SEQ ID NO: 1;
(B) a nucleic acid having the nucleotide sequence of SEQ ID NO: 10; or
(C) a nucleic acid capable of hybridizing with the nucleic acid according to (A) or (B), or a nucleic acid fully complementary thereto, under stringent conditions which include incubation at 50° C. for 12 to 20 hours together with a nucleic acid probe in 6×SSC, wherein 1×SSC has 0.15M NaCl, 0.01 5M sodium citrate and a pH of 7.0 and contains 0.5% by weight of SDS, 0.1% by weight of bovine serum albumin, 0.1% by weight polyvinyl pyrrolidone, 0.1% by weight of a synthetic, water soluble, nonionic copolymer of sucrose and epichlorhydrin having a molecular weight of about 400,000, and 0.01% by weight of denatured salmon sperm DNA.
3. A vector comprising the nucleic acid of claim 2 .
4. An isolated transformant selected from the group consisting of insect cells, animal cells, bacteria, yeasts and fungi resulting from transformation with the vector of claim 3 .
5. A pharmaceutical composition comprising the antigenic polypeptide of claim 1 .
6. A diagnostic composition comprising the antigenic polypeptide of claim 1 .
7. A pharmaceutical composition comprising the nucleic acid of claim 2 .
8. A diagnostic composition comprising the nucleic acid of claim 2 .
9. An isolated nucleic acid capable of specifically binding to the nucleic acid of claim 2 .
10. The antigenic polypeptide of claim 1 which is isolated from fungi other than Saccharomyces cerevisiae.
11. The antigenic polypeptide of claim 10 which is isolated from the fungi belonging to the genus Candida.
12. The nucleic acid of claim 2 which is isolated from fungi other than Saccharomyces cerevisiae.
13. The nucleic acid of claim 12 which is isolated from the fungi belonging to the genus Candida.
14. The antigenic polypeptide of claim 1 , wherein said stringent conditions further include subsequent washing initiated at a temperature of 37° C. in 2×SSC containing 0.5% by weight of SDS, the SSC concentration being variable up to a range of 0.1×SDS and the temperature being variable up to a range of 50° C.
15. The isolated nucleic acid of claim 2 , wherein said stringent conditions further include subsequent washing initiated at a temperature of 37° C. in 2×SSC containing 0.5% by weight of SDS, the SSC concentration being variable up to a range of 0.1×SDS and the temperature being variable up to a range of 50° C.