Bioengineered tissue constructs and methods for producing and using thereof
View Patent ↗Cultured tissue constructs comprising cultured cells and endogenously produced extracellular matrix components without the requirement of exogenous matrix components or network support or scaffold members. Some tissue constructs of the invention are comprised of multiple cell layers or more than one cell type. The tissue constructs of the invention have morphological features and functions similar to tissues their cells are derived and their strength makes them easily handleable. Preferred cultured tissue constructs of the invention are prepared in defined media, that is, without the addition of chemically undefined components.
1. A method for producing a cultured tissue construct, comprising,
(a) seeding human fibroblast cells capable of synthesizing an extracellular matrix on a porous membrane, wherein the membrane comprises pores that are about 3 microns or less in size, in a culture vessel in a chemically defined cell culture medium, wherein the chemically defined cell culture medium is free of undefined animal organ or tissue extracts and comprises the following:
(i) nutrient base medium;
(ii) insulin;
(iii) L-glutamine or an L-glutamine derivative; and
(iv) ascorbate or an ascorbate derivative;
(b) stimulating the fibroblast cells, which are at least at 80% confluence to synthesize, secrete and organize extracellular matrix components; and,
(c) continued culturing of the fibroblast cells to thereby produce a cultured tissue construct with an extracellular matrix of at least about 30 microns thick and cultured fibroblast cells contained within the synthesized extracellular matrix layer, wherein the extracellular matrix comprises:
(i) fibrillar collagen showing a packing organization of fibrils and fibril bundles exhibiting a quarter-staggered 67 nm banding pattern;
(ii) tenascin; and,
(iii) glycosaminoglycans;
and wherein said extracellular matrix is produced by the cultured fibroblast cells on one surface of the porous membrane.
2. The method of claim 1 , wherein the human fibroblast cells are dermal cells.
3. The method of claim 1 , wherein the ascorbate derivative is selected from the group consisting of sodium ascorbate, ascorbic acid and L-ascorbic acid phosphate magnesium salt n-hydrate.
4. The method of claim 1 , wherein the nutrient base medium is selected from the group consisting of Dulbecco's Modified Eagle's Medium (DMEM), Minimal Essential Medium (MEM), M199, RPMI 1640, Iscove's Modified Dulbecco's Medium (EDMEM), Ham's F-12, Ham's F-10, NCTC 109, and NCTC 135 nutrient base media.
5. The method of claim 1 , wherein the L-glutamine derivative is L-alanyl-L-glutamine.