Nucleic acid encoding pyruvate carboxylase from coryneform glutamicum
View Patent ↗A pyruvate carboxylase gene having SEQ ID NO:1 as well as an amino acid sequence having SEQ ID NO:2 expressed by the pyruvate carboxylate gene are disclosed. Also disclosed is a method for microbial production of amino acids of the aspartate and/or glutamate family in which the pyruvate carboxylase activity is increased by genetically changing the pyruvate carboxylase gene having SEQ ID NO:1 through expression of a microorganism which produces the corresponding amino acid having SEQ ID NO:2.
1. A pVWEX1pyc vector contained in the bacterium deposited under DSM 12893.
2. A bacterium comprising the vector of claim 1 .
3. A method of microbially producing L-amino acids of aspartate and/or glutamate families, which comprises the step of:
culturing an amino acid producing microorganism strain in a medium suitable for the production of an L-amino acid, whereby said amino acid producing microorganism strain is transformed with the vector pVWEX1pyc encoding the polypeptide comprising the amino acid sequence having SEQ ID NO:2 with pyruvate carboxylase activity.
4. The method of microbially producing L-amino acids as defined in claim 3 wherein the L-amino acid is selected from the group consisting of L-lysine, L-threonine, L-homoserine and L-glutamate.
5. The method of microbially producing L-amino acids as defined in claim 3 wherein said amino acid producing microorganism strain is Corynebacterium glutamicum.