Variable rate particle counter and method of use
View Patent ↗A variable rate particle counter for adjusting the volumetric delivery rate of fluid to a flow cell based upon an initial particle count rate in order to effectively “tune” the final dilution of sample sheath flow to the particle concentration of the sample. A sheath fluid syringe pump and a test sample syringe pump are driven by motors which are adjusted by a data analyzer. The data analyzer compares a particle count rate measured by a detection assembly to a predetermined reference value and determines if the count rate is too high or to low. Accordingly, one of several pump profiles is initiated to adjust the flow rate of the sheath fluid or test sample or both. Advantageously, the low cell count precision is improved and the upper limit cell count is expanded.
1. A method for improving the precision in counting the number of particles or cells suspended in a given volume of a test sample wherein the number of particles or cells ranges from low particle/cell counts to high particle/cell counts, comprising:
a) delivering a sheath stream of the test sample at a first volumetric flow rate to a particle counting means to count the number of particles or cells in the test sample, wherein the sheath stream has a cross-sectional diameter adapted to deliver to said particle counting means substantially one particle or cell of the test sample at a time;
b) making an initial count at the first volumetric flow rate of the particles or cells of said test sample per unit time with said particle counting means;
c) comparing the initial count of the number of cells or particles in the test sample to a reference value;
d) adjusting the flow rate of the test sample to a second volumetric flow rate based on the comparative number of cells or particles in the test sample to the reference value, thereby improving the precision of the particle counting means in counting the number of cells or particles in the test sample.
2. The method of claim 1 , wherein the second volumetric flow rate optimizes the ability of the detection means to make a precise count of the particles or cells in the test sample.
3. The method of claim 1 , wherein the test sample is a hematology sample.
4. The method of claim 1 , wherein the first volumetric flow rate of the sheath fluid is at laminar flow.
5. The method of claim 1 , wherein the detection means is optical.
6. The method of claim 1 , wherein the detection means is a laser beam.