DNA-construct for the tissue-specific expression of a blood coagulation factor
View Patent ↗A DNA-construct has been disclosed which is suitable for a tissue-specific expression of a blood coagulation factor like Factor IX, which comprises a DNA coding for an amino acid sequence of a blood coagulation factor and a DNA coding for a promoter which is specific for the expression in hematopoietic cells.
1. A DNA-construct for the tissue-specific expression of a blood coagulation factor comprising a DNA sequence coding for an amino acid sequence of a blood coagulation factor and a DNA sequence functioning as a promoter which is specific for expression in megakaryocytes, wherein the DNA sequence functioning as a promoter is the DNA sequence for the human platelet glycoprotein IIb (GPIIb) promoter.
2. The DNA-construct as claimed in claim 1 , wherein the blood coagulation factor is Factor IX.
3. The DNA-construct as claimed in claim 1 , wherein a first truncated intron (Intron 1) of the human FIX gene is inserted into the DNA sequence coding for an amino acid sequence of a blood coagulation factor.
4. A process for the production of a blood coagulation factor in a megakaryocyte cell line comprising:
transfecting megakaryocyte cells with a DNA-construct coding for a blood coagulation factor comprising:
a DNA sequence coding for the amino acid sequence of a blood coagulation factor, and
a DNA sequence functioning as a promoter which is specific for expression in megakaryocytes; and
expressing the DNA-construct.
5. The process as claimed in claim 4 , wherein the production of the blood coagulation factor is stimulated by an inducer.
6. The process as claimed in claim 5 , wherein the inducer is phorbol-12-myristate-13-acetate (PMA).
7. The process according to claim 4 wherein the DNA sequence functioning as a promoter is the DNA sequence for the human platelet glycoprotein IIb (GPIIb) promoter.
8. The process according to claim 4 , wherein the blood coagulation factor is Factor IX.
9. The process according to claim 4 , wherein a first truncated intron (Intron 1) of the human FIX gene is inserted into the DNA sequence coding for an amino acid sequence of a blood coagulation factor.