Means and methods for monitoring protease inhibitor antiretroviral therapy and guiding therapeutic decisions in the treatment of HIV/AIDS
View Patent ↗This invention relates to antiviral drug susceptibility and resistance tests to be used in identifying effective drug regimens for the treatment of human immunodeficiency virus (HIV) infection and acquired immunodeficiency syndrome (AIDS), particularly treatment regimens including a protease inhibitor. The invention further relates to the means and methods of monitoring the clinical progression of HIV infection and its response to antiretroviral therapy using phenotypic or genotypic susceptibility assays.
1. A method for assessing the effectiveness of amprenavir therapy in an HIV-infected patient comprising determining whether a biological sample from said HIV-infected patient contains a nucleic acid encoding HIV protease having a mutation at codon 88, wherein the presence of said nucleic acid in said sample indicates an increase in susceptibility to amprenavir, thereby assessing the effectiveness of amprenavir therapy in the patient.
2. The method of claim 1 , wherein said mutation at codon 88 encodes serine (S).
3. The method of claim 1 , wherein said HIV-infected patient is being treated with an antiretroviral agent.
4. A method for assessing the effectiveness of nelfinavir, indinavir and amprenavir therapy in an HIV-infected patient comprising determining whether a biological sample from said HIV-infected patient contains a nucleic acid encoding HIV protease having a mutation at codon 88 and a mutation at codon(s) 63 and/or 77, wherein the presence of said nucleic acid in said sample indicates a decrease in susceptibility to nelfinavir and indinavir and an increase in susceptibility to amprenavir, thereby assessing the effectiveness of nelfinavir, indinavir, and amprenavir therapy.
5. The method of claim 4 , wherein said mutation at codon 63 encodes proline (P) or glutamine (Q) and said mutation at codon 77 encodes isoleucine (I).
6. The method of claim 4 , wherein said HIV-infected patient is being treated with an antiretroviral agent.
7. A method for assessing the effectiveness of nelfinavir, indinavir and amprenavir therapy in an HIV-infected patient comprising determining whether a biological sample from said HIV-infected patient contains a nucleic acid encoding HIV protease having a mutation at codon 88 and a mutation at codon 63, 77, or 46, or a combination thereof, wherein the presence of said nucleic acid in said sample indicates a decrease in susceptibility to nelfinavir and indinavir and an increase in susceptibility to amprenavir, thereby assessing the effectiveness of nelfinavir, indinavir and amprenavir therapy.
8. The method of claim 7 , wherein said mutation at codon 63 encodes proline (P) or glutamine (Q), said mutation at codon 77 encodes isoleucine (I), and said mutation at codon 46 encodes leucine (L) or isoleucine (I).
9. The method of claim 7 , wherein said HIV-infected patient is being treated with an antiretroviral agent.
10. A method for assessing the effectiveness of nelfinavir, indinavir and amprenavir therapy in an HIV-infected patient, comprising determining whether a biological sample from said HIV-infected patient contains nucleic acid encoding HIV protease having a mutation at codon 88 and a mutation at codon(s) 63, 77, 46, 10, 20 or 36, or a combination thereof, wherein the presence of said nucleic acid in said sample indicates a decrease in susceptibility to nelfinavir and indinavir and an increase in susceptibility to amprenavir, thereby assessing the effectiveness of nelfinavir, indinavir, and amprenavir therapy.
11. The method of claim 10 , wherein said mutation at codon 63 encodes proline (P) or glutamine (Q); said mutation at codon 77 encodes isoleucine (I); said mutation at codon 46 encodes leucine (L) or isoleucine (I); said mutation at codon 10 encodes isoleucine (I) or a phenylalanine (F); said mutation at codon 20 encodes threonine (T), methionine (M), or arginine (R); and said mutation at codon 36 encodes isoleucine (I) or valine (V).
12. The method of claim 10 , wherein said HIV-infected patient is being treated with an antiretroviral agent.