Glyphosate tolerant plants
View Patent ↗Genes encoding a glyphosate oxidoreductase enzyme are disclosed. The genes are useful in producing transformed bacteria and plants which degrade glyphosate herbicide as well as crop plants which are tolerant to glyphosate herbicide.
1. An isolated double-stranded DNA molecule which hybridizes to the DNA sequence of SEQ ID NO:3, wherein said DNA molecule encodes a glyphosate oxidoreductase enzyme.
2. A recombinant bacterium containing the double-straded DNA molecule of claim 1 .
3. The DNA molecule of claim 1 , wherein said DNA molecule comprises SEQ ID NO:17.
4. A An isolated DNA molecule comprising SEQ ID NO:4.
5. An isolated DNA molecule that is capable of hybridizing to the DNA sequence of SEQ ID NO:4, wherein said DNA molecule encodes a glyphosate oxireductase enzyme.
6. A bacterium containing the DNA molecule of claim 5 .
7. A An isolated DNA molecule comprising SEQ ID NO:6.
8. An isolated DNA molecule that is capable of hybridizing to the DNA sequence of SEQ ID NO:6, wherein said DNA molecule encodes a glyphosate oxidoreductase enzyme.
9. A bacterium containing the DNA molecule of claim 8 .
10. A An isolated DNA molecule comprising SEQ ID NO:7.
11. An isolated DNA molecule that is capable of hybridizing to the DNA sequence of SEQ ID NO:7, wherein said DNA molecule encodes a glyphosate oxidoreductase enzyme.
12. A bacterium containing the DNA molecule of claim 11 .
13. A An isolated DNA molecule comprising SEQ ID NO:8.
14. An isolated DNA molecule that is capable of hybridizing to the DNA sequence of SEQ ID NO:8, wherein said DNA molecule encodes a glyphosate oxidoreductase enzyme.
15. A bacterium containing the DNA moledule of claim 14 .
16. A An isolated DNA molecule comprising SEQ ID NO:17.
17. An isolated DNA molecule that is capable of hybridizing to the DNA sequence of SEQ ID NO:17, wherein said DNA molecule encodes a glyphosate oxidoreductase enzyme.
18. A bacterium containing the DNA molecule of claim 17 .
19. A method for selecting transformed plant cells comprising:
(a) introducing a chimeric gene comprising SEQ ID NO:3, 4, 6, 7, 8 or 17 into plant cells;
(b) placing said plant cells on a plant growth media containing glyphosate; and
(c) selecting plant cells that exhibit growth on said glyphosate containing media.
20. A method for selecting transformed plant cells comprising:
(a) introducing a chimeric gene comprising a DNA molecule encoding a glyphosate oxidoreductase enzyme into plant cells, wherein the DNA molecule is capable of hybridizing to SEQ ID NO:3, 4, 6, 7, 8 or 17;
(b) placing said plant cells on a plant growth media containing glyphosate; and
(c) selecting plant cells that exhibit growth on said glyphosate containing media.