Macromolecular arrays on polymeric brushes and methods for preparing the same
View Patent ↗Polymeric brush substrates and methods for their preparation are provided. Methods are also provided for preparing macromolecular arrays on such polymeric brush substrates. Using polymeric brush substrates allows control over functional site density as well as wettability and porosity of the substrate. These polymeric brushes are useful in solid-phase synthesis of arrays of peptides, polynucleotides or small organic molecules.
1. A method of preparing a polymeric brush substrate having a plurality of polynucleotides thereon for use in a polynucleotide array, the method comprising:
(a) providing a substrate to which one or more free radical initiators are covalently attached, wherein each free radical initiator has a radical generation site distal to the substrate;
(b) contacting the covalently attached substrate with monomers under conditions that promote free radical polymerization from the radical generation sites of the initiators to form a polymeric brush; and
(c) covalently attaching a plurality of polynucleotides to a plurality of reactive groups on the polymeric brush.
2. The method of claim 1 , wherein step (b) comprises living free radical polymerization.
3. The method of claim 1 , wherein the substrate comprises glass or silica.
4. The method of claim 1 , wherein the monomers comprise a vinyl group.
5. The method of claim 1 , wherein the polymer brush formed on the support comprises hydroxyl, amino, carboxyl, or sulfydryl groups or a combination thereof.
6. The method of claim 1 , wherein the monomers comprise vinyl acetate.