Transgenic plants with a modified activity of a plastidial ADP/ATP translocator
View Patent ↗Transgenic plant cells and plants are described which, compared to wildtype cells and plants, exhibit an increased yield, in particular, an increased oil and/or starch content and which preferably synthesize a modified starch. The described plants exhibit an increase or a decrease of the plastidial ADP/ATP translocator activity.
1. A genetically modified plant cell wherein a foreign nucleic molecule encoding a plastidial ADP/ATP translocator is integrated into the nuclear genome of said genetically modified plant cell and wherein the expression of said foreign nucleic acid molecule results in an increase in plastidial ADP/ATP translocator activity in comparison with corresponding non-genetically modified plant cells from wild type plants.
2. The genetically modified plant cell according to claim 1 exhibiting an increased starch content in comparison with corresponding non-genetically modified plant cells.
3. The genetically modified plant cell according to claim 1 synthesizing a starch fraction exhibiting an increased amylose-content in comparison with a starch fraction from corresponding non-genetically modified plant cells.
4. A genetically modified plant containing transgenic plant cells according to claim 1 .
5. The genetically modified plant according to claim 4 , which is a maize, wheat or potato plant.
6. A method for the production of a transgenic plant exhibiting an increased yield in comparison with wild type plants, wherein
(a) a plant cell is genetically modified by integrating a foreign nucleic acid molecule encoding a plastidial ADP/ATP translocator into the nuclear genome of said plant cell wherein the expression of said foreign nucleic acid molecule results in an increase in plastidial ADP/ATP translocator activity in the cell;
(b) a plant is regenerated from the cell produced according to step (a); and
(c) further transgenic plants are optionally produced from the plant produced according to step (b).
7. The method according to claim 6 , wherein the transgenic plant exhibits (a) an increased starch content in comparison with wild type plants, (b) a starch fraction with an increased amylose content in comparison with a starch fraction from the wild type plants, or (c) both an increased starch content in comparison with wild type plants and a starch fraction with an increased amylose content in comparison with starch friction from wild type plants.
8. A transgenic plant obtainable by the method according to claim 6 or 7 .
9. Propagation material of genetically modified plants according to any one of claim 4 or 5 , wherein the propagation material has ADP/ATP translocator activity.
10. A method for the production of a modified starch comprising the extraction of the starch from a plant according to any one of claim 4 or 5 .
11. The genetically modified plant cell according to claim 1 exhibiting an increased starch content in comparison with corresponding non-genetically modified plant cells.
12. The method according to claim 6 , wherein the transgenic plant exhibits an increased starch content in comparison with wild type plants and a starch fraction with an increased amylose content in comparison with a starch fraction from wild type plants.
13. The method according to claim 6 , wherein the transgenic plant exhibits an increased starch content in comparison with wild type plants or a starch fraction with an increased amylose content in comparison with a starch fraction from wild type plants.
14. A transgenic plant produced by the method according to claim 6 .
15. A genetically modified plant cell wherein a foreign nucleic molecule encoding an Arabidopsis thaliana, Solanum tuberosum, Rickettsia prowazekii or Chlamydia trachomatis plastidial ADP/ATP translocator is integrated into the nuclear genome of said genetically modified plant cell and wherein the expression of said foreign nucleic acid molecule results in an increase in plastidial ADP/ATP translocator activity in comparison with corresponding non-genetically modified plant cells from wild type plants.
16. A genetically modified plant cell wherein a foreign nucleic molecule encoding a plastidial ADP/ATP translocator protein having an amino acid sequences of SEQ ID NO. 5, SEQ ID NO. 6 or SEQ ID NO. 7 is integrated into the nuclear genome of said genetically modified plant cell and wherein the expression of said foreign nucleic acid molecule results in an increase in plastidial ADP/ATP translocator activity in comparison with corresponding non-genetically modified plant cells from wild type plants.
17. A method for the production of a transgenic plant exhibiting an increased starch, increased amylose, or increased starch and amylose in comparison with wild type plants, wherein
(a) a plant cell is genetically modified by integrating a foreign nucleic acid molecule encoding an Arabidopsis thaliana, Solanum tuberosum, Rickettsia prowazekii or Chlamydia trachomatis plastidial ADP/ATP translocator into the nuclear genome of said plant cell wherein the expression of said foreign nucleic acid molecule results in an increase in plastidial ADP/ATP locator activity in the cell;
(b) a plant is regenerated from the cell produced according to step (a); and
(c) further transgenic plants are optionally produced from the plant produced according to step (b).
18. A method for the production of a transgenic plant exhibiting an increased starch, increased amylose, or increased starch and amylose in comparison with wild type plants, wherein
(a) a plant cell is genetically modified by integrating a foreign nucleic acid molecule encoding a plastidial ADP/ATP translocator protein having an amino acid sequence of SEQ ID NO. 5, SEQ ID NO. 6 or SEQ ID NO. 7 into the nuclear genome of said plant cell wherein the expression of said foreign nucleic acid molecule results in an increase in plastidial ADP/ATP translocator activity in the cell;
(b) a plant is regenerated from the cell produced according to step (a); and
(c) further transgenic plants are optionally produced from the plant produced according to step (b).
19. A method for the production of a transgenic plant exhibiting an increased starch, increased amylose, or increased starch and amylose in comparison with wild type plants, wherein
(a) a plant cell is genetically modified by integrating a foreign nucleic acid molecule encoding a plastidial ADP/ATP translocator protein whose amino acid sequence is at least 66% homologous to SEQ ID NO. 5 into the nuclear genome of said plant cell wherein the expression of said foreign nucleic acid molecule results in an increase in plastidial ADP/ATP translocator activity in the cell;
(b) a plant is regenerated from the cell produced according to step (a); and
(c) further transgenic plants are optionally produced from the plant produced according to step (b).
20. A genetically modified plant cell wherein a foreign nucleic molecule encoding a plastidial ADP/ATP translocator protein whose amino acid sequence is at least 66% homologous to SEQ ID NO. 5 is integrated into the nuclear genome of said genetically modified plant cell and wherein the expression of said foreign nucleic acid molecule results in an increase in plastidial ADP/ATP translocator activity in comparison with corresponding non-genetically modified plant cells from wild type plants.