IP Library › Granted Patent US 6,933,112
Granted Patent B1
US 6,933,112 · App. 09/675,518 · Granted Aug 23, 2005

Device for mass transport assisted optical assays

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Quick Facts
Patent No.
US 6,933,112
App. No.
09/675,518
Granted
Aug 23, 2005
Kind
B1
Abstract

An optical assay device for the detection of an analyte of interest in a sample comprising a support containing channels, an optically functional layer positioned on the support such that the optically functional layer and the support allow for laminar flow of the sample through layers of the device, an attachment layer positioned on the optically functional layer, and an analyte specific receptive layer positioned on the attachment layer.

Claims (36)

1. A support comprising a surface on which an assay for an analyte of interest can be performed, comprising:

an attachment layer comprising diamond-like carbon having sp 2 and sp 3 character on the support surface, wherein the attachment layer is adapted for capture of the analyte of interest for detection in the assay by binding the analyte directly to the diamond-like carbon; and

an optically functional layer, an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, positioned on said support.

2. A support according to claim 1 , wherein the attachment layer comprises a layer of diamond-like carbon of between about 50 Å to about 3000 Å in thickness.

3. A support according to claim 1 , wherein the attachment layer has a degree of hydrophobicity resulting from a preselected sp 2 and sp 3 character of the diamond-like carbon.

4. A support according to claim 1 , wherein the diamond-like carbon is configured to function as an antireflective layer.

5. A support according to claim 1 , wherein the optically functional layer is interposed between the support surface and the attachment layer.

6. A support according to claim 1 , wherein the support provides a change in optical thickness upon binding of the analyte capable of attenuating one or more wavelengths of light.

7. A support according to claim 1 , wherein the support provides laminar flow through or across the support.

8. A support according to claim 1 , wherein the attachment layer comprises diamond-like carbon in a form selected from the group consisting of amorphous carbon with diamond-like hardness and surface energy properties, amorphous hydrogenated diamond-like carbon, and non-crystalline carbon films with diamond-like hardness and surface energy properties.

9. A support according to claim 1 , wherein the diamond-like carbon comprises non-carbon material.

10. A support according to claim 9 , selected from the group consisting of hydrogen, silicon, and nitrogen.

11. A support according to claim 1 , wherein the support comprises a material that is not compatible with high temperatures.

12. A support according to claim 11 , wherein said high temperature is greater than 100° C.

13. A support according to claim 11 , wherein the material that is not compatible with high temperatures is selected from the group consisting of cellulose acetate, polyethylene terephthalate (PETE), polyester, polycarbonate, nylon, filter paper, polysulfones, polypropylene, and polyurethane.

14. A support according to claim 11 , wherein the diamond like carbon has a hardness of about 15 to about 50 Gpa.

15. A support according to claim 11 , wherein the attachment layer has a refractive index of about 1.5 to about 2.2.

16. A support according to claim 1 , wherein said support is a biosensor.

17. A support according to claim 1 , wherein said attachment layer comprises diamond-like carbon in a form selected from the group consisting of amorphous carbon with diamond-like hardness and surface energy properties, amorphous hydrogenated diamond-like carbon, and non-crystalline carbon films with diamond-like hardness and surface energy properties.

18. A support comprising a surface on which an assay for an analyte of interest can be performed, comprising:

an attachment layer comprising a layer of diamond-like carbon having sp 2 and sp 3 character of between about 50 Å to about 500 Å in thickness on the support surface, wherein said attachment layer comprises a capture molecule bound to said diamond-like carbon for specific capture of said analyte by binding said analyte to said capture molecule.

19. A support according to claim 18 , wherein said capture molecule is selected from the group consisting of an antigen, an antibody, a receptor, a nucleic acid, an RNA, a DNA, a polysaccharide, a lipopolysaccharide, an enzyme, a protein, a microorganism, a hapten, a drug, a ligand, and a chelator.

20. A support according to claim 18 , wherein attachment layer has a degree of hydrophobicity resulting from a preselected sp 2 and s 3 character of the diamond-like carbon.

21. A support according to claim 18 , wherein said diamond-like carbon is an antireflective layer.

22. A support according to claim 18 , wherein the optically functional layer is interposed between said surface and said attachment layer.

23. A support according to claim 18 , wherein said support provides a change in optical thickness upon binding of said analyte capable of attenuating one or more wavelengths of light.

24. A support according to claim 18 , wherein said support provides laminar flow through or across said support.

25. A support according to claim 18 , wherein said attachment layer comprises diamond-like carbon in a form selected from the group consisting of amorphous carbon with diamond-like hardness and surface energy properties, amorphous hydrogenated diamond-like carbon, and non-crystalline carbon films with diamond-like hardness and surface energy properties.

26. A support according to claim 18 , wherein the diamond-like carbon comprises non-carbon material.

27. A support according to claim 26 , wherein the non-carbon material is selected from the group consisting of hydrogen, silicon, and nitrogen.

28. A support according to claim 18 , wherein the support comprises a material that is not compatible with high temperatures.

29. A support according to claim 28 , wherein said high temperature is greater than 100° C.

30. A support according to claim 28 , wherein the material that is not compatible with high temperatures is selected from the group consisting of cellulose acetate, polyethylene terephthalate (PETE), polyester, polycarbonate, nylon, filter paper, polysulfones, polypropylene, and polyurethane.

31. A support according to claim 28 , wherein the diamond like carbon has a hardness of about 15 to about 50 Gpa.

32. A support according to claim 28 , wherein the attachment layer has a refractive index of about 1.5 to about 2.2.

33. A support according to claim 18 , wherein said support is a biosensor.

Assignments (8)
NOTICE OF RELEASE OF SECURITY INTEREST IN PATENTS RECORDED AT REEL 026557 FRAME 0287 Recorded Jun 23, 2015
From: GENERAL ELECTRIC CAPITAL CORPORATION, AS ADMINISTRATIVE AGENT AND COLLATERAL AGENT
To: ADVANTAGE DIAGNOSTICS CORPORATION; ALERE MEDICAL, INC.; ALERE SAN DIEGO, INC.; ALERE SCARBOROUGH, INC.; AMEDITECH INC.; APPLIED BIOTECH, INC.; BINAX, INC.; BIOSITE INCORPORATED; CHOLESTECH CORPORATION; GENECARE MEDICAL GENETICS CENTER, INC.; HEMOSENSE, INC.; INSTANT TECHNOLOGIES, INC.; INVERNESS MEDICAL - BIOSTAR INC.; ISCHEMIA TECHNOLOGIES, INC.; MATRITECH, INC.; MATRIA HEALTHCARE, INC.; ZYCARE, INC.
Reel/Frame 036011/0581 →
SECURITY AGREEMENT Recorded Jul 7, 2011
From: ADVANTAGE DIAGNOSTICS CORPORATION; ALERE MEDICAL INCORPORATED; ALERE SAN DIEGO, INC.; ALERE SCARBOROUGH, INC.; AMEDITECH INC.; APPLIED BIOTECH, INC.; BINAX, INC.; BIOSITE INCORPORATED; CHOLESTECH CORPORATION; GENECARE MEDICAL GENETICS CENTER, INC.; HEMOSENSE, INC.; INSTANT TECHNOLOGIES, INC.; INVERNESS MEDICAL - BIOSTAR INC.; ISCHEMIA TECHNOLOGIES, INC.; MATRITECH, INC.; ZYCARE INC.; MARTIA HEALTHCARE, INC.
To: GENERAL ELECTRIC CAPITAL CORPORATION
Reel/Frame 026557/0287 →
CORRECTIVE ASSIGNMENT TO CORRECT THE INCORRECT APPLICATION NO. 09/375,518 PREVIOUSLY RECORDED ON REEL 012189 FRAME 0506. ASSIGNOR(S) HEREBY CONFIRMS THE CHANGE OF NAME. Recorded May 20, 2010
From: BIOSTAR, INC.
To: THERMO BIOSTAR INC.
Reel/Frame 024415/0478 →
SECOND LIEN INTELLECTUAL PROPERTY SECURITY AGREEMENT Recorded Jun 27, 2007
From: IM US HOLDINGS, LLC, A DELAWARE LIMITED LIABILITY COMPANY; INVERNESS MEDICAL INNOVATIONS, INC., A DELAWARE CORPORATION; ADVANTAGE DIAGNOSTICS CORPORATION, A DELAWARE CORPORATION; APPLIED BIOTECH, INC., A CALIFORNIA CORPORATION; BINAX, INC., A DELAWARE CORPORATION; FIRST CHECK DIAGNOSTICS CORP., A DELAWARE CORPORATION; FOREFRONT DIAGNOSTICS, INC., A CALIFORNIA CORPORATION; INCA ACQUISITION, INC., A DELAWARE CORPORATION; INNOVATIVE MOBILITY, LLC, A FLORIDA LIMITED LIABILITY COMPANY; INSTANT TECHNOLOGIES, INC., A VIRGINIA CORPORATION; INVERNESS MEDICAL - BIOSTAR, INC., A DELAWARE CORPORATION; INVERNESS MEDICAL INTERNATIONAL HOLDING CORP., A DELAWARE CORPORATION; INVERNESS MEDICAL INTERNATIONAL HOLDING CORP. II, A DELAWARE CORPORATION; INNOVATIONS RESEARCH, LLC, A DELAWARE LIMITED LIABILITY COMPANY; INVERNESS MEDICAL, LLC, A DELAWARE LIMITED LIABILITY COMPANY; ISCHEMIA TECHNOLOGIES, INC., A DELAWARE CORPORATION; IVC INDUSTRIES, INC., A DELAWARE CORPORATION; INNOVACON, INC., A DELAWARE CORPORATION; OSTEX INTERNATIONAL, INC., A WASHINGTON CORPORATION; QUALITY ASSURED SERVICES, INC., A FLORIDA CORPORATION; SELFCARE TECHNOLOGY, INC., A DELAWARE CORPORATION; SPDH, INC., A DELAWARE CORPORATION; UNIPATH ONLINE, INC., A MASSACHUSETTS CORPORATION; WAMPOLE LABORATORIES, LLC, A DELAWARE LIMITED LIABILITY COMPANY
To: GENERAL ELECTRIC CAPITAL CORPORATION, AS AGENT
Reel/Frame 019477/0808 →
FIRST LIEN INTELLECTUAL PROPERTY SECURITY AGREEMENT Recorded Jun 26, 2007
From: IM US HOLDINGS, LLC, A DELAWARE LIMITED LIABILITY COMPANY; INVERNESS MEDICAL INNOVATIONS, INC., A DELAWARE CORPORATION; ADVANTAGE DIAGNOSTICS CORPORATION, A DELAWARE CORPORATION; APPLIED BIOTECH, INC., A CALIFORNIA CORPORATION; BINAX, INC., A DELAWARE CORPORATION; FIRST CHECK DIAGNOSTICS CORP., A DELAWARE CORPORATION; FOREFRONT DIAGNOSTICS, INC., A CALIFORNIA CORPORATION; INCA ACQUISITION, INC., A DELAWARE CORPORATION; INNOVATIVE MOBILITY, LLC, A FLORIDA LIMITED LIABILITY COMPANY; INSTANT TECHNOLOGIES, INC., A VIRGINIA CORPORATION; INVERNESS MEDICAL - BIOSTAR, INC., A DELAWARE CORPORATION; INVERNESS MEDICAL INTERNATIONAL HOLDING CORP., A DELAWARE CORPORATION; INVERNESS MEDICAL INTERNATIONAL HOLDING CORP. II, A DELAWARE CORPORATION; INNOVATIONS RESEARCH, LLC, A DELAWARE LIMITED LIABILITY COMPANY; INVERNESS MEDICAL, LLC, A DELAWARE LIMITED LIABILITY COMPANY; ISCHEMIA TECHNOLOGIES, INC., A DELAWARE CORPORATION; IVC INDUSTRIES, INC., A DELAWARE CORPORATION; INNOVACON, INC., A DELAWARE CORPORATION; OSTEX INTERNATIONAL, INC., A WASHINGTON CORPORATION; SELFCARE TECHNOLOGY, INC., A DELAWARE CORPORATION; SPDH, INC., A DELAWARE CORPORATION; UNIPATH ONLINE, INC., A MASSACHUSETTS CORPORATION; QUALITY ASSURED SERVICES, INC., A FLORIDA CORPORATION
To: GENERAL ELECTRIC CAPITAL CORPORATION, AS AGENT
Reel/Frame 019477/0268 →
CHANGE OF NAME Recorded Oct 18, 2006
From: THERMO BIOSTAR, INC.
To: INVERNESS MEDICAL - BIOSTAR INC.
Reel/Frame 018398/0869 →
SECURITY AGREEMENT Recorded Sep 28, 2006
From: INVERNESS MEDICAL -BIOSTAR INC. (F/K/A THERMO BIOSTAR INC.)
To: GENERAL ELECTRIC CAPITAL CORPORATION, AS AGENT
Reel/Frame 018338/0227 →
CHANGE OF NAME Recorded Sep 24, 2001
From: BIOSTAR, INC.
To: THERMO BIOSTAR INC.
Reel/Frame 012189/0506 →