IP Library › Granted Patent US 6,846,658
Granted Patent B1
US 6,846,658 · App. 09/689,343 · Granted Jan 25, 2005

Method for cloning and producing the Msel restriction endonuclease

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Quick Facts
Patent No.
US 6,846,658
App. No.
09/689,343
Granted
Jan 25, 2005
Kind
B1
Abstract

A method for cloning restriction-modification system is provided whereby the target modification methylase is produced and confers full protection during all growth phases in which the cognate restriction enzyme is present. The method is employed in the cloning of the MseI restriction-modification system.

Claims (6)

1. An isolated DNA coding for MseI restriction endonuclease, wherein the isolated DNA is obtainable from ATCC No. PTA-2421.

2. A recombinant DNA vector comprising the isolated DNA of claim 1 .

3. An isolated DNA coding for MseI restriction endonuclease and methylase, wherein the isolated DNA is obtainable from ATCC No. PTA-2421.

4. A recombinant DNA vector which comprises the isolated DNA of claim 3 .

5. A host cell transformed by the vector of claim 2 or 4 .

6. A method of producing the MseI restriction endonmuclease comprising, culturing a host cell transformed with the vector of claim 2 or 4 under conditions suitable for expression of said endonuclease.

Assignments (1)
TERMINATION AND RELEASE OF SECURITY INTEREST IN PATENTS Recorded Sep 20, 2023
From: BANK OF AMERICA, N.A. (AS SUCCESSOR-BY-MERGER WITH FLEET NATIONAL BANK)
To: NEW ENGLAND BIOLABS, INC.
Reel/Frame 064962/0066 →