Coding sequences of the human BRCA1 gene
View Patent ↗isolated coding sequences and to the protein sequences they code for. This invention is directed to three coding sequence of the BRCA1 gene. The three coding sequences, BRCA1 (omi1) , BRCA1 (omi2) , and BRCA1 (omi3) and their frequencies of occurrence are provided together with the protein sequences they code for. Another aspect of this invention is a method of determining the consensus sequence for any gene. Another aspect of the invention is a method of identifying an individual having an increased genetic susceptibility to breast or ovarian cancer because they have inherited a causative mutation in their BRCA1 gene. This invention is also related to a method of performing gene therapy with any of the isolated BRCA1 coding sequences.
1. An isolated nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 3.
2. The isolated nucleic acid molecule of claim 1 , wherein the nucleic acid consists essentially of the nucleotide sequence of SEQ ID NO: 3.
3. The isolated nucleic acid molecule of claim 1 , wherein the nucleic acid molecule consists of the nucleotide sequence of SEQ ID NO: 3.
4. The isolated nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises nucleotides 120 to 5,708 of SEQ ID NO: 3.
5. The isolated nucleic acid molecule of claim 1 , wherein the nucleic acid molecule consists of nucleotides 120 to 5,708 of SEQ ID NO: 3.
6. The isolated nucleic acid molecule of claim 1 , wherein the nucleic acid molecule is operably linked to one or more expression control elements.
7. A vector comprising an isolated nucleic acid molecule of claim 1 .
8. A host cell comprising the vector of claim 7 .
9. A host cell transformed to contain the nucleic acid molecule of claim 1 .
10. A host cell of claim 8 or 9 , wherein said host is selected from the group consisting of prokaryotic hosts and eukaryotic hosts.
11. A method for producing a polypeptide comprising culturing a host cell of claim 8 or 9 under in vitro conditions in which the protein encoded by the nucleic acid molecule is expressed.
12. A method for producing a polypeptide comprising culturing a host cell containing a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 3 under in vitro conditions in which the protein encoded by the nucleic acid molecule is expressed.
13. The method of claim 12 , wherein the nucleic acid consists essentially of the nucleotide sequence of SEQ ID NO: 3.
14. The method of claim 12 , wherein the nucleic acid molecule consists of the nucleotide sequence of SEQ ID NO: 3.
15. The method of claim 12 , wherein the nucleic acid molecule comprises nucleotides 120 to 5,708 of SEQ ID NO: 3.
16. The method of claim 12 , wherein the nucleic acid molecule consists of nucleotides 120 to 5,708 of SEQ ID NO: 3.