A rapid, simple-to-use method for preparing hybrid cells, applicable to fully differentiate, non-dividing cells, entails bringing at least two different cells into contact under conditions that promote cell fusion and then purifying the resultant hybrid without antibiotic or metabolic selection. This approach yields hybrid cells useful in a variety of applications, including clinical treatment regimens, as cellular modulators of the immune system.
1. A method of preparing a composition for cancer immunotherapy, comprising:
(a) staining a neoplastic cell population freshly isolated from a patient with a first fluorescent dye,
(b) staining a dendritic cell population with a second fluorescent dye, wherein said first dye is different from said second dye,
(c) contacting said neoplastic cell population and said dendritic cell population with one another under conditions that promote cell fusion, and obtaining a resultant hybrid cell population stained with both the first and second dyes,
(d) purifying the resultant hybrid cell population by fluorescence activated cell sorting, and
(e) resuspending the resultant hybrid cell population in a pharmaceutically acceptable vehicle;
wherein said method does not involve antibiotic or metabolic selection, said purifying is accomplished in less than about 24 to 48 hours after said contacting, and the antigenic diversity of the starting neoplastic cell population is preserved in the resultant hybrid cell population.
2. The method of claim 1 wherein the resultant hybrid cell population contains less than 10% of its total population as reactant cells.
3. The method of claim 1 , wherein the resultant hybrid cell population contains less than 5% of its total population as reactant cells.
4. The method of claim 1 , wherein said pharmaceutically acceptable vehicle is normal saline.
5. A method of preparing a hybrid cell preparation, comprising:
(a) staining a tumor cell population freshly isolated from a patient with a first fluorescent dye,
(b) staining a dendritic cell population with a second fluorescent dye, wherein said first dye is different from said second dye,
(c) contacting said tumor cell population and said dendritic cell population with one another under conditions that promote cell fusion, and obtaining a resultant hybrid cell population stained with both the first and second dyes, and
(d) purifying the resultant hybrid cell population by fluorescence activated cell sorting,
wherein said method does not involve antibiotic or metabolic selection, said purifying is accomplished in less than about 24 to 48 hours after said contacting, and the antigenic diversity of the starting tumor cell population is preserved in the resultant hybrid cell population.
6. The method of claim 5 , further comprising:
(e) resuspending the resultant hybrid cell population in a pharmaceutically acceptable buffer.
7. The method of claim 5 wherein the resultant hybrid cell population contains less than 10% of its total population as reactant cells.
8. The method of claim 5 , wherein the resultant hybrid cell population contains less than 5% of its total population as reactant cells.
9. The method of claim 6 , wherein said pharmaceutically acceptable buffer is normal saline.
10. The method of claim 5 , wherein said first and second fluorescent dyes are endotoxin-free, pyrogen-free or both.
11. The method of claim 5 , wherein said tumor cell is a primary tumor cell.
12. The method of claim 1 , wherein said first and second fluorescent dyes are endotoxin-free, pyrogen-free or both.
13. The method of claim 1 , wherein said neoplastic cell is a tumor cell.
14. The method of claim 13 , wherein said tumor cell is a primary tumor cell.