Genes differentially expressed in breast cancer
View Patent ↗A polynucleotide sequence as shown in SEQ ID NO:1 is associated with metastatic potential of cancer cells, especially breast cancer cells. Methods are provided for determining the risk of metastasis of a tumor, by determining whether a tissue sample from a tumor expresses a polypeptide or mRNA encoded by a polynucleotide as shown in SEQ ID NO:1. Also provided are therapeutic methods and compositions.
1. An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
(a) a polynucleotide encoding amino acids from 1 to 273 of SEQ ID NO:2;
(b) a polynucleotide encoding amino acids from 2 to 273 of SEQ ID NO:2;
(c) a polynucleotide encoding amino acids from 26 to 273 of SEQ ID NO:2; and
(d) the polynucleotide complement of the complete polynucleotide of (a), (b), or (c).
2. An isolated nucleic acid molecule comprising a polynucleotide encoding a polypeptide wherein, except for no more than 5 conservative amino acid substitutions, said polypeptide has an amino acid sequence selected from the group consisting of:
(a) amino acids 1 to 273 of SEQ ID NO:2;
(b) amino acids 2 to 273 of SEQ ID NO:2; and
(c) amino acids 26 to 273 of SEQ ID NO:2.
3. The isolated nucleic acid molecule of claim 1 , which is DNA.
4. A method of making a recombinant vector comprising inserting a nucleic acid molecule of claim 1 ( a ), ( b ), or ( c ), into a vector in operable linkage to a promoter.
5. A recombinant vector produced by the method of claim 4 .
6. A method of making a recombinant host cell comprising introducing the recombinant vector of claim 5 into a host cell.
7. A recombinant host cell produced by the method of claim 6 .
8. A recombinant method of producing a polypeptide, comprising culturing the recombinant host cell of claim 7 under conditions such that said polypeptide is expressed and recovering said polypeptide.
9. An isolated nucleic acid molecule comprising a polynucleotide at least 95% identical to a polynucleotide selected from the group consisting of:
(a) a polynucleotide encoding amino acids from 1 to 273 of SEQ ID NO:2;
(b) a polynucleotide encoding amino acids from 2 to 273 of SEQ ID NO:2;
(c) a polynucleotide encoding amino acids from 26 to 273 of SEQ ID NO:2; and
(d) the full polynucleotide complement of the complete polynucleotide of (a), (b), or (c).
10. The isolated nucleic acid molecule of claim 9 wherein the polynucleotide is at least 98% identical to the polynucleotide of (a)-(d).
11. A method of making a recombinant vector comprising inserting a nucleic acid molecule of claim 9 into a vector in operable linkage to a promoter.
12. An isolated nucleic acid molecule comprising a polynucleotide encoding a polypeptide at least 95% identical to SEQ ID NO:2, or the full complement of the complete polynucleotide.
13. The isolated nucleic acid molecule of claim 12 wherein the polynucleotide encodes a polypeptide at least 98% identical to SEQ ID NO:2.
14. The isolated nucleic acid molecule of any one of claims 2 , 9 or 12 wherein the polynucleotide is at least 95% identical to SEQ ID NO:1.
15. The isolated nucleic acid molecule of any one of claims 2 , 9 or 12 wherein the polynucleotide is at least 98% identical to SEQ ID NO:1.
16. The isolated nucleic acid molecule of claim 2 wherein the polynucleotide encodes a polypeptide wherein, except for no more than 3 conservative amino acid substitutions, said polypeptide has an amino acid sequence selected from the group consisting of:
(a) amino acids 1 to 273 of SEQ ID NO:2;
(b) amino acids 2 to 273 of SEQ ID NO:2; and
(c) amino acids 26 to 273 of SEQ ID NO:2.
17. The isolated nucleic acid molecule of any one of claims 2 , 9 or 12 wherein the nucleic acid molecule encodes a polypeptide comprising SEQ ID NO:10.
18. The isolated nucleic acid molecule of any one of claims 2 , 9 or 12 wherein the nucleic acid molecule encodes a polypeptide comprising SEQ ID NO:3.
19. The isolated nucleic acid molecule of claim 2 comprising a polynucleotide encoding a polypeptide having no more than 5 conservative amino acid substitutions, wherein the conservative substitutions are selected from the group consisting of:
(a) leucine to isoleucine;
(b) leucine to valine;
(c) aspartate to glutamate; and
(d) threonine to serine.