IP Library › Granted Patent US 7,112,330
Granted Patent B1
US 7,112,330 · App. 09/762,762 · Granted Sep 26, 2006

Method for producing yeast expressed HPV types 6 and 16 capsid proteins

View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 7,112,330
App. No.
09/762,762
Granted
Sep 26, 2006
Kind
B1
Abstract

Mosaic VLPs of viral capsid proteins from different virus types are described, as are methods of making the same. Specifically, a diploid yeast strain that coexpresses the L1 and L2 capsid proteins of both HPV-6 and HPV-16 as mosaic VLPs is described. The mosaic VLPs induced the production conformational antibodies against both L1 proteins upon administration to mice.

Claims (17)

1. A virus-like particle (VLP) comprising a first late 1 (L1) capsid protein from human papilloma virus (HPV) type 6 and a second L1 capsid protein from HPV type 16.

2. The VLP of claim 1 , further comprising an L2 capsid protein.

3. A composition comprising the VLP of claim 1 .

4. The composition of claim 3 wherein the VLP is purified for immunization.

5. The composition of claim 4 , wherein the VLP comprises an L2 capsid protein.

6. The composition of claim 5 , further comprising an adjuvant.

7. The composition of claim 6 wherein the adjuvant is MF59.

8. The VLP of claim 1 wherein said VLP induces an immune response against both types of viruses.

9. A composition comprising the VLP of claim 8 .

10. A method for producing the VLP of claim 1 , said method comprising a) cloning said capsid proteins into expression cassettes comprising the same promoters and termination sequences; and b) expressing said cassettes in the same yeast diploid host cell.

11. The method of claim 10 wherein the yeast is Saccharomyces cerevisiae.

12. The method of claim 10 , wherein the capsid proteins further comprise L2 capsid proteins.

13. The method of claim 12 wherein said L1 protein expression cassettes are cloned into non-integrative vectors, and said L2 proteins expression cassettes are cloned into integrative vectors.

14. The method of claim 13 wherein the non-integrative vector is pBS24.1.

15. The method of claim 13 wherein the integrative vector is pUC8.

16. A method for inducing an immune response against more than one type of virus comprising administering the VLP of claim 1 .

17. A method for inducing an immune response against more than one type of virus comprising administering the composition of claim 5 .