Multiparallel three dimensional optical microscopy system
View Patent ↗Volume imaging is supported in an optical apparatus that simultaneously acquires multiple images from multiple focal planes in an image object. A plurality of image detectors is arranged with respect to an objective lense. A plurality of reflective devices are disposed between the objective lense and the plurality of detectors to divide the light travelling through the objective lense to the detectors into channels such that each channel is registered with a respective detector.
1. An optical apparatus for simultaneously acquiring multiple images from multiple focal planes in an image object, comprising:
an objective lense;
a plurality of image detectors, each image detector having a respective optical displacement with respect to the objective lense so as to acquire images from differing focal planes;
a plurality of reflective devices disposed between the objective lense and the plurality of image detectors and arranged to divide light travelling through the objective lense toward the plurality of detectors into a plurality of channels, each channel registered with a respective image detector; and
a plurality of magnification correction optics, each magnification correction optics disposed between the plurality of reflective devices and a respective image detector to correct magnification of the respective image detector.
2. The optical apparatus of claim 1 , wherein each channel is an optical path for transmission of identical image information from the objective lense to the respective image detector.
3. The optical apparatus of claim 1 , each image detector of the plurality of image detectors including a line array device.
4. The optical apparatus of claim 3 , further including a sample holder for supporting and moving a sample in synchronism with line scanning of the plurality of line array devices.
5. An optical apparatus for simultaneously acquiring multiple images from a three-dimensional sample volume, comprising:
an objective lense;
a plurality of image detectors, each image detector having a respective optical displacement with respect to the objective lense so as to acquire images from focal planes positioned at different depths within the three-dimensional sample volume;
a plurality of reflective devices disposed between the objective lense and the plurality of image detectors and arranged to divide light travelling through the objective lense toward the plurality of detectors into a plurality of channels, each channel registered with a respective image detector; and
a plurality of magnification correction optics, each magnification correction optics disposed between the plurality of reflective devices and a respective image detector to correct magnification of the respective image detector.
6. The optical apparatus of claim 5 , wherein each channel is an optical path for transmission of identical image information from the objective lense to the respective image detector.
7. The optical apparatus of claim 5 , each image detector of the plurality of image detectors including a line array device.
8. The optical apparatus of claim 7 , further including a sample holder for supporting and moving a sample in synchronism with line scanning of the plurality of line array devices.
9. An optical apparatus for acquiring multiple images from a three-dimensional sample volume, comprising:
an objective lense;
a plurality of image detectors, each image detector having a respective optical displacement with respect to the objective lense so as to simultaneously acquire images from differing focal planes;
a plurality of reflective devices disposed between the objective lense and the plurality of image detectors and arranged to divide light travelling through the objective lense toward the plurality of detectors into a plurality of channels, each channel registered with a respective image detector; and
a plurality of magnification correction optics, each magnification correction optics disposed between the plurality of reflective devices and a respective image detector to correct magnification of the respective image detector.
10. The optical apparatus of claim 9 , wherein each channel is an optical path for transmission of identical image information from the objective lense to the respective image detector.
11. The optical apparatus of claim 9 , each image detector of the plurality of image detectors including a line array device.
12. The optical apparatus of claim 11 , further including a sample holder for supporting and moving a sample in synchronism with line scanning of the plurality of line array devices.