Isolated human ras-like proteins, nucleic acid molecules encoding these human ras-like proteins, and uses thereof
View Patent ↗The present invention provides amino acid sequences of polypeptides that are encoded by genes within the human genome, the Ras-like protein polypeptides of the present invention. The present invention specifically provides isolated polypeptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the Ras-like protein polypeptides, and methods of identifying modulators of the Ras-like protein polypeptides.
1. An isolated nucleic acid molecule consisting of a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence that encodes a protein comprising the amino acid sequence of SEQ ID NO:2;
(b) a nucleotide sequence consisting of SEQ ID NO:1;
(c) a nucleotide sequence consisting of SEQ ID NO:3; and
(d) a nucleotide sequence that is completely complementary to a nucleotide sequence of (a)-(c).
2. An isolated polynucleotide consisting of a nucleotide sequence set forth in SEQ ID NO:1.
3. An isolated polynucleotide consisting of a nucleotide sequence set forth in SEQ ID NO:3.
4. A nucleic acid vector comprising a nucleic acid molecule of claim 1 .
5. A vector according to claim 4 , wherein said vector is selected from the group consisting of a plasmid, virus, and bacteriophage.
6. A vector according to claim 4 , wherein said isolated nucleic acid molecule is inserted into said vector in proper orientation and correct reading frame such that the protein of SEQ ID NO:2 may be expressed by a cell transformed with said vector.
7. A vector according to claim 6 , wherein said isolated nucleic acid molecule is operatively linked to a promoter sequence.
8. An isolated host cell containing the vector of claim 4 .
9. A process for producing a polypeptide comprising culturing the host cell of claim 8 under conditions sufficient for the production of said polypeptide, and recovering said polypeptide from the host cell culture.
10. A method for detecting the presence of a nucleic acid molecule of claim 1 in a sample, said method comprising
contacting the sample with an oigonucleotide comprising at least 20 contiguous nucleotides that hybridizes to said nucleic acid molecule under stringent conditions, wherein the stringent condition is hybridization in 6× sodium chloride/sodium citrate (SSC) at about 45° C., followed by one or more washes in 0.2×SCC, 0.1% SDS at 50-65° C., and
determining whether the oligonucleotide binds to said nucleic acid molecule in the sample.