Nucleic acid encoding KCNB potassium channel
View Patent ↗The present invention provides nucleic acid and protein sequences for a novel potassium channel protein, KCNB. The herein-disclosed sequences can be used for any of a number of purposes, including for the specific detection of KCNB, for the identification of molecules that associate with and/or modulate the activity of KCNB, to diagnose any of a number of conditions associated with KCNB or KCNB activity, or to modulate the number or activity of KCNB molecules in a mammal.
1. An isolated nucleic acid encoding a potassium channel polypeptide that transduces a change in membrane potential, wherein the polypeptide comprises 90% or greater identity to the amino acid sequence of SEQ ID NO:1.
2. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises greater than 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO:1.
3. The isolated nucleic acid of claim 1 , wherein the nucleic acid encodes a polypeptide that specifically binds to polyclonal antibodies generated against an amino acid sequence of SEQ ID NO:1.
4. An isolated nucleic acid encoding a protein comprising an amino acid sequence of SEQ ID NO:1.
5. An isolated nucleic acid comprising a nucleotide sequence of SEQ ID NO:2 or the protein coding region set forth in SEQ ID NO:5.
6. The isolated nucleic acid of claim 1 , wherein the nucleic acid is amplified by a primer pair selected from the group consisting of SEQ ID NO:6 and SEQ ID NO:7; and SEQ ID NO:9 and SEQ ID NO:10.
7. An isolated nucleic acid encoding a potassium channel polypeptide that transduces a change in membrane potential and comprises 90% or greater identity to the amino acid sequence of SEQ ID NO:1, wherein the nucleic acid selectively hybridizes under stringent hybridization conditions comprising 50% formamide, 5×SSC and 1% SDS at 42° C. and wash conditions comprising 0.2×SSC and 0.1% SDS at 65° C. to a nucleotide sequence of SEQ ID NO:2 or SEQ ID NO:5.
8. An isolated nucleic acid encoding a potassium channel polypeptide that transduces a change in membrane potential, wherein the polypeptide comprises at least 200 contiguous amino acids of the amino acid sequence of SEQ ID NO:1.
9. An isolated nucleic acid of claim 8 , wherein the polypeptide comprises two pore domains and four transmembrane domains as set forth in FIG. 1 .
10. An expression vector comprising the nucleic acid of claim 1 , claim 4 , claim 5 , claim 7 , or claim 8 .
11. A host cell transfected with the vector of claim 10 .
12. A method of making a potassium channel polypeptide, the method comprising the step of expressing the polypeptide from a recombinant expression vector comprising a nucleic acid encoding the polypeptide, wherein the amino acid sequence of the polypeptide comprises 90% or greater amino acid identity to SEQ ID NO:1 and the encoded polypeptide transduces a change in membrane potential.
13. A method of making a recombinant cell comprising a potassium channel polypeptide, the method comprising the step of transducing the cell with an expression vector comprising a nucleic acid encoding the polypeptide, wherein the amino acid sequence of the polypeptide comprises 90% or greater amino acid identity to SEQ ID NO:1 and the encoded polypeptide transduces a change in membrane potential.
14. An isolated nucleic acid, or complement thereof, comprising at least 50 contiguous nucleotides of SEQ ID NO:2 or SEQ ID NO:5.
15. An isolated nucleic acid, or complement thereof, comprising at least 100 contiguous nucleotides of SEQ ID NO:2 or SEQ ID NO:5.