Wuschel (wus) gene homologs
View Patent ↗This invention relates to an isolated nucleic acid fragment encoding a WUS protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the WUS protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the WUS protein in a transformed host cell.
1. An isolated polynucleotide comprising:
a nucleotide sequence encoding the WUSCHEL polypeptide of SEQ ID NO: 6 or SEQ ID NO: 8 which stimulates in vitro growth of plant tissue.
2. The isolated polynucleotide of claim 1 , wherein the nucleotide sequence comprises SEQ ID NO:5, or SEQ ID NO:7.
3. An isolated polynucleotide comprising the complement of the polynucleotide of any one of claims 1 and 2 , wherein the complement and the polynucleotide consist of the same number of nucleotides and are 100% complementary.
4. A chimeric gene comprising the polynucleotide of any one of claims 1 and 2 , operably linked to a regulatory sequence.
5. A transgenic plant comprising the polynucleotide of claim 1 .
6. The transgenic plant of claim 5 , wherein the plant is corn, soybean, wheat, rice, alfalfa, sunflower, canola, or cotton.
7. A seed from the transgenic plant of claim 5 , wherein the seed comprises the polynucleotide.
8. The seed of claim 7 , wherein the seed is from corn, soybean, wheat, rice, alfalfa, sunflower, canola, or cotton.
9. A method for inducing meristem proliferation in a plant cell comprising:
(a) transforming a plant cell with the polynucleotide of claim 1 operably linked to a regulatory sequence operable in the plant cell; and,
(b) expressing the polynucleotide to induce meristem proliferation.
10. The method of claim 9 wherein the polynucleotide is integrated into the plant cell genome to produce a transformed plant cell comprising the polynucleotide.
11. The method of claim 10 further comprising growing the transformed plant cell under plant growing conditions to produce a regenerated plant.
12. A plant produced by the method of claim 11 , wherein the plant comprises the polynucleotide.
13. The plant of claim 12 , wherein the plant is corn, soybean, wheat, rice, alfalfa, sunflower, canola, or cotton.
14. A method for positive selection of a transformed cell, comprising:
(a) transforming a plant cell with the polynucleotide of claim 1 operably linked to a regulatory sequence operable in the plant cell, and
(b) expressing the polynucleotide for a time sufficient to induce organogenesis and provide a positive selection means.
15. A method for transforming a plant cell comprising introducing the polynucleotide of any one of claims 1 and 2 into the cell.
16. The transformed plant cell produced by the method of claim 15 , wherein the plant cell comprises the polynucleotide.
17. A method for transforming a plant cell comprising introducing the polynucleotide of claim 3 into the cell.
18. The transformed plant cell produced by the method of claim 17 , wherein the plant cell comprises the polynucleotide.
19. The method of claim 15 , further comprising growing the transformed plant cell under plant growing conditions to produce a regenerated plant.
20. The method of claim 17 , further comprising growing the transformed plant cell under plant growing conditions to produce a regenerated plant.
21. A transformed plant produced by the method of claim 9 , wherein the plant comprises the polynucleotide.
22. A transformed plant produced by the method of claim 20 , wherein the plant comprises the polynucleotide.
23. The plant of claim 21 , wherein the plant is corn, soybean, wheat, rice, alfalfa, sunflower, canola, or cotton.
24. The plant of claim 22 , wherein the plant is corn, soybean, wheat, rice, alfalfa, sunflower, canola, or cotton.