Isolated human ras-like proteins, nucleic acid molecules encoding these human ras-like proteins, and uses thereof
View Patent ↗The present invention provides amino acid sequences of polypeptides that are encoded by genes within the human genome, the Ras-like protein polypeptides of the present invention. The present invention specifically provides isolated polypeptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the Ras-like protein polypeptides, and methods of identifying modulators of the Ras-like protein polypeptides.
1. An isolated nucleic molecule consisting of a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence consisting of SEQ ID NO:1;
(b) a nucleotide sequence consisting of SEQ ID NO:3; and
(c) a nucleotide sequence that is completely complementary over the entire length of a nucleic acid molecule of (a) or (b).
2. A nucleic acid construct comprising the nucleic acid molecule of claim 1 , used to a heterologous nucleotide sequence.
3. The nucleic acid construct of claim 2 , wherein the heterologous nucleotide sequence encodes a heterologous polypeptide.
4. A vector comprising the nucleic acid molecule of claim 1 .
5. The vector of claim 4 , wherein said isolated nucleic acid molecule is inserted into said vector in proper orientation and correct reading frame such that a polypeptide may be expressed by a cell transformed with said vector, wherein the amino acid sequence of said polypeptide consists of SEQ ID NO:2.
6. The vector of claim 5 , wherein said isolated nucleic acid molecule is operatively linked to a promoter sequence.
7. An isolated host cell containing the vector of claim 4 .
8. A process for producing a polypeptide wherein the amino acid sequence of said polypeptide consists of SEQ ID NO:2, the process comprising culturing the host cell of claim 7 under conditions sufficient for the production of said polypeptide, and recovering said polypeptide, thereby producing said polypeptide.
9. The vector of claim 4 , wherein said vector is selected from the group consisting of a plasmid, a virus, and a bacteriophage.
10. An isolated polynucleotide consisting of the nucleotide sequence of SEQ ID NO:1.
11. An isolated polynucleotide consisting of the nucleotide sequence of SEQ ID NO:3.