Isolated human Ras-like proteins, nucleic acid molecules encoding these human Ras-like proteins, and uses thereof
View Patent ↗The present invention provides amino acid sequences of polypeptides that are encoded by genes within the human genome, the Ras-like protein polypeptides of the present invention. The present invention specifically provides isolated polypeptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the Ras-like protein polypeptides, and methods of identifying modulators of the Ras-like protein polypeptides.
1. An isolated nucleic acid molecule consisting of a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2;
(b) a nucleotide sequence consisting of SEQ ED NO:1;
(c) a nucleotide sequence consisting of SEQ ED NO:3; and
(d) a nucleotide sequence that is completely complementary over the entire length of a nucleotide sequence of (a)-(c).
2. A vector comprising the nucleic acid molecule of claim 1 .
3. The vector of claim 2 , wherein said vector is selected from the group consisting of a plasmid, a virus, and a bacteriophage.
4. The vector of claim 2 , wherein said isolated nucleic acid molecule is inserted into said vector in proper orientation and correct reading frame such that a polypeptide comprising SEQ ID NO:2 may be expressed by a cell transformed with said vector.
5. The vector of claim 4 , wherein said isolated nucleic acid molecule is operatively linked to a promoter sequence.
6. A host cell containing the vector of claim 2 .
7. A process for producing a polypeptide comprising the amino acid sequence of SEQ ID NO:2, the process comprising culturing the host cell of claim 6 under conditions sufficient for the production of said polypeptide, and recovering said polypeptide, thereby producing said polypeptide.
8. An isolated polynucleotide consisting of the nucleotide sequence of SEQ ID NO:1.
9. An isolated polynucleotide consisting of the nucleotide sequence of SEQ ID NO:3.